@Article{Stanilov2016,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="20",
number="6",
year="2016",
title="Increased transforming growth factor β and interleukin 10 transcripts in peripheral blood mononuclear cells of colorectal cancer patients",
abstract=" Aim of the study:  The ability of immune cells in peripheral blood to produce certain cytokines affects tumour-elicited inflammation. The aim of this study was to investigate the gene expression of interleukin 12A (IL-12A), IL-12B, IL-23A, IL-10, IL-6, transforming growth factor β (TGF-β), HDAC3, and iNOS in peripheral blood mononuclear cells (PBMC) from colorectal cancer (CRC) patients.    Material and methods:  The venous blood for PBMC isolation was collected preoperatively and 10 days after surgery, from CRC patients. After isolation of total RNA and synthesis of cDNA, quantitative real-time PCR assays were performed.    Results : Our results demonstrated that among investigated cytokine genes IL-10 and TGF-b were significantly upregulated in patients with CRC compared to the control group, while the expression of IL-23 mRNA was significantly decreased in CRC patients. We observed significantly increased mRNA levels in CRC patients’ PBMC before surgery for IL-10 and TGF-b compared to both postoperative and control groups. We also found a significant upregulation of iNOS in early compared to advanced CRC.   Conclusions : Based on the results we can assume that PBMC gene expression programming in CRC patients drives local differentiation of Th cells towards Treg instead of the Th1 anti-tumour subpopulation.",
author="Stanilov, Noyko S.
and Miteva, Lyuba
and Cirovski, Geo
and Stanilova, Spaska A.",
pages="458--462",
doi="10.5114/wo.2016.65605",
url="http://dx.doi.org/10.5114/wo.2016.65605"
}