@Article{Zhang2017,
journal="Archives of Medical Science",
issn="1734-1922",
volume="13",
number="6",
year="2017",
title="Value of CNRIP1 promoter methylation in colorectal cancer screening and prognosis assessment and its influence on the activity of cancer cells",
abstract=" Introduction : The aim of the study was to investigate the effect of  CNRIP1  promoter methylation on the proliferative, invasive and migration potential of colorectal cancer cells, including its potential use for the early detection and prognostic assessment of colorectal cancer.    Material and methods : Quantitative methylation-specific PCR (qMSP) was used to detect the methylation status of the  CNRIP1  promoter region in peripheral blood samples drawn from patients with colorectal adenocarcinoma, benign colorectal adenoma, and matched healthy controls. Putative CpG methylation sites were then pyrosequenced. We subsequently suppressed  CNRIP1  methylation within colon cancer cells via treatment with 5-azacytidine and overexpressed colon cancer cells by transfection with a  CNRIP1 -overexpression pcDNA3.0 plasmid. Thereafter, the  CNRIP1  methylation status and mRNA and protein expressions levels were determined. Finally, the proliferative, invasive and migration abilities of cell lines were determined with the CCK-8 and Transwell cell assays.   Results:   There were differences in the methylation status at loci 2216, 2226, 2231, 2245, and 2254 within the promoter region of  CNRIP1  between patients with colorectal adenocarcinoma, colorectal adenoma, and healthy volunteers. The methylation status of CpG sequence 2245 significantly correlated with tumor diameter, invasion depth, TNM stage, grade, and lymph node metastasis (p < 0.05). The proliferative, invasive and migration abilities of colon cancer cells treated with 5-azaC or transfected with a  CNRIP1 -overexpression plasmid were significantly impaired relative to negative controls (p < 0.05).    Conclusions : The methylation status at locus 2245 within the  CNRIP1  promoter region has potential value for the early detection and prognostic evaluation of colorectal cancers. Demethylation of the  CNRIP1  promoter or overexpression of  CNRIP1  can reduce the proliferative and migration abilities of colon cancer cells.",
author="Zhang, Ting
and Cui, Ge
and Yao, Yun-Liang
and Wang, Qi-Chun
and Gu, Hong-Guang
and Li, Xi-Ning
and Zhang, Hui
and Feng, Wen-Ming
and Shi, Qi-Lin
and Cui, Weiwei",
pages="1281--1294",
doi="10.5114/aoms.2017.65829",
url="http://dx.doi.org/10.5114/aoms.2017.65829"
}