@Article{Gambichler2017,
journal="Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii",
issn="1642-395X",
volume="34",
number="4",
year="2017",
title="Cutaneous leukocytoclastic vasculitis: the roleof lymphocytes and related immune markers",
abstract="  Introduction:   Apart from neutrophils, other immune cells may play a significant pathogenetic role in cutaneous leukocytoclastic vasculitis (CLV).     Aim:    To investigate lymphocytes and related immunological factors in patients with CLV requiring systemic glucocorticosteroid treatment.    Material and methods:    Fourteen patients with severe idiopathic CLV were treated with systemic prednisolone in a tapered dose regimen. Ten healthy individuals served as controls. At baseline and post-treatment, we studied inducer/helper and suppressor/cytotoxic T lymphocytes, B lymphocytes, natural killer cells, CD4+CD25++CD127– cells, CD4+CD25+CD39+ cells and FOXP3, transforming growth factor 1 (TGF-1) and interleukin-10 (IL-10) mRNA levels in the blood using flow cytometry and real time polymerase chain reaction (RT-PCR), respectively. On immunohistochemistry, we studied CD4, CD8, granzyme B, TGF-1, and IL-10.    Results:   Flow cytometry did not show significant differences. The RT-PCR revealed that TGF-1 mRNA expression was significantly higher after therapy when compared to baseline and controls. On immunohistology, baseline CLV lesions showed significantly more CD4+ lymphocytes than post-treated CLV and controls. CD8+ expression was significantly higher after therapy when compared to baseline and controls. Baseline granzyme B was significantly increased when compared to treated CLV and controls. The IL-10 expression of treated CLV was significantly increased when compared to baseline CLV and; baseline CLV IL-10 expression was significantly increased as compared to controls.    Conclusions:    Circulating T regulatory cells do not play a significant role in the pathogenesis of CLV. T helper cells and granzyme B seem to be involved in the inflammatory cutaneous process of CLV. A resolution of CLV observed after glucocorticosteroid treatment may be mediated via up-regulation of TGF-1 and IL-10 in different compartments.",
author="Gambichler, Thilo
and Kulik, Magdalena A.
and Skrygan, Marina
and Rooms, Isabelle
and Höxtermann, Stefan",
pages="299--305",
doi="10.5114/ada.2017.69307",
url="http://dx.doi.org/10.5114/ada.2017.69307"
}