@Article{Al-Majali2017,
journal="Central European Journal of\ Immunology",
issn="1426-3912",
volume="42",
number="3",
year="2017",
title="Immunomodulatory effect of Moringa peregrina leaves, ex vivo and in vivo study",
abstract="This study was conducted to assess the     in vivo     and  ex vivo  immunomodulatory effect of the ethanol leaves extract of  Moringa peregrina  in Balb/c mice. For this study, five groups of 5 Balb/c mice were given a single acute subtoxic oral dose of the ethanolic extract at 1.13, 11.30, 23.40 and 113.4 mg/kg and the immunomodulatory effect was assessed on the 6th day following the ingestion. In the (non-functional) assessment, the effect of the extract on the body weight, relative lymphoid organ weight, splenic cellularity and peripheral blood hematologic parameters were evaluated. While in the immunomodulation assessment (functional), we investigated the effect of the extract on the proliferative capacity of splenic lymphocytes and peripheral T and B lymphocytes using mitogen blastogenesis, mixed allogeneic MLR and IgM-Plaque forming cells assays. The ingestion of  M. peregrina  extract caused a significant increase in the body weight, weight and number of cells of spleen and lymph nodes of the treated mice. Furthermore, the count of RBCs, WBCs, platelets, hemoglobin concentration and PCV % were increased by the extract treatment in a dose-dependent manner.  M. peregrina  enhanced the proliferative responses of splenic lymphocytes for both T cell and B-cell mitogens. Likewise, the mixed lymphocyte reaction MLR assay has revealed a T-cell dependent proliferation enhancement in the extract treated mice. Moreover, the oral administration of  M. peregrina  leaves extracts significantly increased PFCs/10 6  splenocytes in a dose-dependent manner. In conclusion, subtoxic acute doses of  M. peregrina  extract demonstrated significant potential as an immunomodulatory agent even at the lowest dose of 1.13 mg/kg.",
author="Al-Majali, Ibrahim Salameh
and Al-Oran, Sawsan Atallah
and Hassuneh, Mona Rushdie
and Al-Qaralleh, Haitham Naief
and Rayyan, Walid Abu
and Al-Thunibat, Osama Yosef
and Mallah, Eyad
and Abu-Rayyan, Ahmed
and Salem, Shadi",
pages="231--238",
doi="10.5114/ceji.2017.70964",
url="http://dx.doi.org/10.5114/ceji.2017.70964"
}