@Article{Padzik2017,
journal="Klinika Oczna / Acta Ophthalmologica Polonica",
issn="0023-2157",
volume="119",
number="2",
year="2017",
title="Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin",
abstract=" Introduction :  Acanthamoebic keratitis , detected with increasing frequency worldwide in the last decades, mainly is reported in contact lens wearers.  	Other risk factors include damage to corneal cells, eye surgery and ocular exposure to water containing developmental forms of the amphizoic amoebae. In this study we analyzed  Acanthamoeba  isolates from complicated keratitis and evaluated some corneal strains in terms of their in vitro susceptibility to toyocamycin.  	 Material and methods : Six strains of  Acanthamoebic keratitis  in contact lens wearers, one with a history of swimming in a lake, previously improperly diagnosed and treated were analyzed.  Acanthamoeba  corneal isolates of T4 genotype cultured in vitro were monitored.  	 A. polyphaga  corneal strain and environmental  A. castellanii  Neff exposed to toyocamycin, the agent with an established anti-trichomonal and anti-toxoplasmal activity, were evaluated.  	 Results :  A. polyphaga  strain, which is not commonly diagnosed as an etiological agent of  Acanthamoeba  keratitis in Poland, was the most virulent in vitro. Amoebostatic effect of toyocamycin, without stimulation of encystation, was stronger for the corneal strain than for the environmental  A. castellanii  Neff.  	 Conclusions : There is no sufficient knowledge of the epidemiology of  Acanthamoeba  keratitis in Poland. Extremely high antimicrobial resistance of  Acanthamoeba  cysts leads to treatment failure, thus chemicals of possible anti-amoebic activity are still tested. Promising results of our study of adenosine analogue as the agent against  Acanthamoeba  pathogenic strains justifies further tests with this drug and various amphizoic amoebic strains. This was the first study of in vitro susceptibility of corneal  Acanthamoeba  strain to toyocamycin.",
author="Padzik, Marcin
and Szaflik, Jacek P.
and Izdebska, Justyna
and Szostakowska, Beata
and Szaflik, Jerzy
and Chomicz, Lidia",
pages="77--82",
doi="10.5114/ko.2017.71774",
url="http://dx.doi.org/10.5114/ko.2017.71774"
}