@Article{Strosznajder2005,
journal="Folia Neuropathologica",
issn="1641-4640",
volume="43",
number="3",
year="2005",
title="Original articleInhibition of poly(ADP-ribose) polymerase activity protects hippocampal cells against morphological and ultrastructural alteration evoked by ischemia-reperfusion injury",
abstract="Poly(ADP-ribose) polymerase 1 (PARP-1 EC 2.4.2.30) is a nuclear enzyme that plays an important role in cell survival and death. PARP is involved in DNA repair machinery, however, massive DNA damage leads to overactivation of PARP-1 and to depletion of its substrate bNAD +  which causes cell death. Our previous study indicated that the PARP activity was significantly activated during ischemia-reperfusion injury.  In this study we investigated the effect of PARP inhibitor, 3-aminobenzamide (3-AB) on intracellular organelles alteration. Gerbils were submitted to 3 and 10 min transient global ischemia followed by recirculation and survival for 1 till 7 days. The histological and electron microscopic examination indicated a pronounced protective effect of 3-AB on the swelling of astrocytes and neurons 1 day after 3 and 10 min ischemic insult. It decreased also the swelling of pericytes. 3-AB decreases evoked by ischemia swelling of mitochondria and Golgi apparatus. The significant ameliorating effect of 3-AB was also observed on the 7th day of reperfusion after 3 min ischemia and was also visible on the 1st day after 10 min ischemia. However, 7 days after prolonged 10 min ischemia almost all neurons in the CA1 hippocampal layer died and 3-AB was not able to protect these cells. In spite of that, 3-AB markedly decreased immunostaining of glial fibrillary acidic protein (GFAP), which was enhanced in the stratum: oriens, radiatum and lacunosum-moleculare at the 7th day after 10 min ischemia. These data indicated that inhibition of PARP may have a protective effect on neuronal cells affected by ischemia-reperfusion injury.",
author="Strosznajder, Robert Piotr
and Gadamski, Roman
and Walski, Michał",
pages="156--165",
url="https://www.termedia.pl/Original-article-Inhibition-of-poly-ADP-ribose-polymerase-activity-protects-hippocampal-cells-against-morphological-and-ultrastructural-alteration-evoked-by-ischemia-reperfusion-injury,20,3764,1,1.html"
}