@Article{Lee2020,
journal="Polish Journal of Pathology",
issn="1233-9687",
volume="71",
number="2",
year="2020",
title="Preparation of frozen cell-block sections amenableto diagnostic immunocytochemistry:  a technical report on cryo-embedded cell-block method",
abstract="Modified agarose cell-block (CB) technique can be effectively used to have the CB embedded in the OCT compound for the preparation of frozen CB (F-CB) sections in the same way as in the preparation of frozen sections from cryoembedded fresh tissue samples for the intraoperative consultation. In this report, we demonstrate the amenability of F-CB sections to the diagnostic immunocytochemistry. The pelleted cytologic material was at first compactly embedded in ultralow-gelling temperature agarose gel and then re-embedded in conventional agarose gel. The resulting agarose-embedded cell-pellet was cut in halves so that one half is embedded in OCT compound for cryosectioning on a cryostat, while the other half is reserved for the preparation of paraffin-embedded CB (P-CB). The F-CB sections were comparable to sections cut from the paraffin-embedded CB in terms of the quality of H\&E-staining and immunocytochemistry. We suppose this method can also facilitate a rapid quantitative and qualitative assessment of the future P-CB.  We have extended this technique to the cryo-embedded cell-block method, in which the compact agarose cell pellet is directly embedded in the OCT compound so that the frozen sections can be cut from the cryo-embedded cell block in the same way as in intraoperative frozen section analysis. In this technical report, we illustrate how the frozen cell blocks (F-CBs) can be effectively prepared not only from liquid-based cytology samples but also from conventional fine-needle aspiration cytology slides.",
author="Lee, Joo
and Choi, Suk
and Kim, Lucia
and Park, In
and Han, Jee
and Kim, Joon",
pages="146--154",
doi="10.5114/pjp.2020.97022",
url="http://dx.doi.org/10.5114/pjp.2020.97022"
}