@Article{Wątor2023,
journal="Polish Journal of Pathology",
issn="1233-9687",
volume="74",
number="4",
year="2023",
title="No differences in miRNA expression in the stroma  of ERG+ and ERG– prostate cancers",
abstract="Prostate cancer (PC) is one of the most common cancers in males. A significant proportion of PCs bear TMPRSS2-ETS translocation and overexpress ERG transcription factor, allowing classification into ERG+ and ERG– groups, which differ in several features including the tumor microenvironment. The aim of the study was to verify whether they differ in expression of the miRNA in the microenvironment. The material consisted of 150 radical prostatectomies. Immunohistochemistry (IHC) for ERG was done using a routine method. FISH for TMPRSS2-ETS translocation was done with a ZytoLight SPEC ERG/TMPRSS2 TriCheck Probe. From each case, a representative section was selected, and tumor and non-tumor were microdissected with the LMD7000 device. RNA was isolated using the RNeasy Mini Kit system (Qiagen) and miRNA libraries were prepared with the NEBNext Multiplex Small RNA Library Prep Set for Illumina and their sequencing was performed on the NexSeq 500. Statistical analysis was done with Statistica and R software. When analyzing the expression of miRNAs some differences could be seen, but after correction for multiple comparisons was applied, these were found to be non- significant.",
author="Wątor, Gracjan
and Kołton-Wróż, Maria
and Wołkow, Paweł
and Bełch, Łukasz
and Chłosta, Piotr
and Szpor, Joanna
and Klimkowska, Agnieszka
and Nejman, Katarzyna
and Józkowicz, Alicja
and Piechota-Polańczyk, Aleksandra
and Okoń, Krzysztof",
pages="265--270",
doi="10.5114/pjp.2023.134304",
url="http://dx.doi.org/10.5114/pjp.2023.134304"
}