@Article{Megawati2025,
journal="Contemporary Oncology/Współczesna Onkologia",
issn="1428-2526",
volume="29",
number="4",
year="2025",
title="Cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC fruit on B16F10 melanoma cell culture",
abstract="This study aimed to investigate the cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC (ZA) fruit on B16F10 melanoma cell cultures.  Material and methods: The ethyl acetate fraction was obtained through maceration followed by fractionation with n-hexane and ethyl acetate. Cytotoxicity was assessed using the MTT assay to determine the IC50 value. B16F10 melanoma cells were then grouped into control, IC50, ¾ IC50, ½ IC50, and doxorubicin groups. Apoptosis induction was analysed using Annexin V and caspase-3 expression assays.  Results: The ethyl acetate fraction of ZA fruit exhibited cytotoxic activity on B16F10 melanoma cells, with an IC50 value of 249.3 µg/ml. Apoptosis induction was confirmed by a significant increase in caspase-3 expression compared to the control group (p < 0.05).  Conclusions: The ethyl acetate fraction of ZA fruit demonstrated cytotoxic effects and induced apoptosis in B16F10 melanoma cells, potentially through caspase-3 activation.",
author="Megawati, Eka
and Bangun, Hakim
and Putra, Imam
and Rusda, Muhammad",
pages="354--359",
doi="10.5114/wo.2025.155773",
url="http://dx.doi.org/10.5114/wo.2025.155773"
}