@Article{Toka2005,
journal="Central European Journal of\ Immunology",
issn="1426-3912",
volume="30",
number="1",
year="2005",
title="Ectromelia virus persistence revisited: virus detection by in situ PCR after long-term infection of BALB/c mice",
abstract=" We measured the extent of ectromelia virus (ECTV) persistence in experimentally infected BALB/c [H-2 d ] by means of in situ PCR and report that ECTV persists in the spleen for at least 12 weeks. Importantly, ECTV maintained a low profile replication in the spleen of mice allowing it to persist considerably longer following initial infection in the absence of clinical disease. Recovery of the virus by cell culture was possible up to 8 weeks post infection. Since ECTV is an Orthopoxvirus that infects genetically susceptible mouse strains, the implication of our findings is that careful screening for this natural mouse pathogen using sensitive assays such as in situ PCR should be periodically carried out in colonies of susceptible mice. On the other hand ECTV and BALB/c [H-2 d ] form a suitable study model for generalized viral infection. As such in the light of the data described here the model would suit investigations on the immune status of persistently infected animals.  ",
author="Toka, Felix N.
and Irma Spohr, Cespedes
and Schollenberger, Ada
and Krzyżanowska, Małgorzata
and Gieryńska, Małgorzata
and Ru, Miao
and Niemiałtowski, Marek",
pages="1--2",
url="https://www.termedia.pl/Ectromelia-virus-persistence-revisited-virus-detection-by-in-situ-PCR-after-long-term-infection-of-BALB-c-mice,10,6418,1,1.html"
}