@Article{Dziankowska-Bartkowiak2007,
journal="Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii",
issn="1642-395X",
volume="24",
number="2",
year="2007",
title="Original article Serum levels of metalloproteinases-2 and -9 (MMP-2, MMP-9) \– correlation with their expression in skin lesions in systemic sclerosis \– the preliminary examination",
abstract="The role of extracellular matrix metalloproteinases (MMPs) is implicated in pathogenesis of systemic sclerosis (SSc).  The aim of present study was a comparison of serum levels of two metalloproteinases %u2013 gelatinases MMP-2 and MMP-9 with their expression in lesional skin biopsies from patients with systemic sclerosis. 10 patients with systemic sclerosis (6 with limited SSc and 4 with diffuse SSc) and 10 healthy volunteers without any skin disease were examined.  Evaluation of serum levels of MMP-2 and MMP-9 was performed by ELISA method with R\&D Systems. Expression of MMPs was determined by immunohistochemistry in DAKO En Vision detection system using immunoperoxidase method with mouse monoclonal antibodies.  Mean serum levels of MMP-2 were not statistically different between SSc patients and control group or both clinical types of SSc (lSSc 245.2 ng/ml vs. dSSc %u2013 248.0 ng/ml) and control group (246.4 ng/ml vs. 248.0 ng/ml) (p>0.05). Mean serum level of MMP-9 was statistically higher in SSc patients compared to control group (857.7 ng/ml vs. 431.1 ng/ml; p 0.05). Statistically significant negative correlation was observed between serum concentrations of MMP-9 and duration of skin sclerosis (\&#961; = -0.860; p<0.02).  Expression of both examined metalloproteinases was stronger in lesional skin in comparison to control group biopsies.  Obtained results confirm important role of MMPs in the pathogenesis of systemic sclerosis.",
author="Dziankowska-Bartkowiak, Bożena
and Żebrowska, Agnieszka
and Joss-Wichman, Ewa
and Kobos, Józef
and Waszczykowska, Elżbieta",
pages="73--81",
url="https://www.termedia.pl/Original-article-Serum-levels-of-metalloproteinases-2-and-9-MMP-2-MMP-9-8211-correlation-with-their-expression-in-skin-lesions-in-systemic-sclerosis-8211-the-preliminary-examination,7,8187,1,1.html"
}