%0 Journal Article %J Central European Journal of Immunology %@ 1426-3912 %V 38 %N 1 %D 2013 %F Yang2013 %T Experimental immunologyPotential role of RING finger protein 166 (RNF166), a member of an ubiquitin ligase subfamily, involved in regulation of T cell activation %X RING (really interesting new gene) finger protein 166, or RNF166, belongs to a C3HC4 ubiquitin ligases subfamily, which include four related proteins containing a conserved C3HC4 (Cys3-His-Cys4) RING finger domain. RNF125, one member of the subfamily, has been identified as a regulator of T cell activation, but the potential roles of another member RNF166 remains poorly understood. Here we reported that RNF166 is involved in regulation of T cell activation. Flow cytometry (FCM) data showed that overexpression of RNF166 in primary T cells and Jurkat T cells induced over 2-fold increase of CD69, a T-cell activation marker, suggesting that RNF166 is a positive-regulator of T cell activation. Furthermore, pull-down assays showed that RNF166 can bind with both Lys 48 -linked polyubiquitin and Lys 63 -linked polyubiquitin, indicating that RNF166 may play regulating roles in T cell activation by self-degradation via ubiquitin-proteasome pathway and/or cross-talk with certain signaling pathways via non-proteasome-dependent pathways. In conclusion, our work reveals that RNF166 is a potential positive-regulator of T cell activation and these findings provide a novel insight into understanding the functions of RNF166 in the positive regulation of immune responses. %A Yang, Ping %A Lu, Yilu %A Jiang, Xue %A Li, Minhui %A Li, Chao %A Chen, Huijuan %A Zhang, Kun %A Pan, Kejian %A Tao, Dachang %A Zhang, Sizhong %A Ma, Yongxin %P 15-22 %9 journal article %R 10.5114/ceji.2013.34353 %U http://dx.doi.org/10.5114/ceji.2013.34353