%0 Journal Article %J Central European Journal of Immunology %@ 1426-3912 %V 28 %N 3 %D 2003 %F Pałynyczko2003 %T Experimental immunologyThe analysis of L-selectin expression intensity (CD62L) on B-lymphocytes in B-cell chronic lymphocytic leukemia cells (B-CLL) %X Decreased percentage of B cells expressing L-selectin in B-cell chronic lymphocytic leukemia (B-CLL) is known from literature. The purpose of our studies was to compare the intensity of L-selectin expression on subpopulation of L-selectin positive B-lymphocytes in untreated and treated patients with B-CLL. The lymphocytes were obtained from peripheral blood of 70 patients suffering from B-CLL (40 untreated and 30 after the treatment) and 20 healthy blood donors. The percentage of positive cells and intensity of CD62 L-selectin expression (RFI) were measured on CD19+ B lymphocytes using tri-color fluorochrom labeled monoclonal antibodies and flow cytometric analysis. The lower percentage of L-selectin positive B-cells paralleled with considerable decrease of this selectin intensity was observed in patients with B-CLL in comparison to healthy subjects. Both these changes may contribute to the impaired adhesion and migration of B-cells in B-CLL resulting in the accumulation of these cells in the peripheral blood. In a group of patients entirely responding to the treatment the tendency toward normalization of both measured parameters has been observed. %A Pałynyczko, Grażyna %A Kopeć-Szlęzak, Joanna %A Konopka, Lech %A Podstawka, Urszula %P 105-109 %9 journal article %U https://www.termedia.pl/Experimental-immunology-The-analysis-of-L-selectin-expression-intensity-CD62L-on-B-lymphocytes-in-B-cell-chronic-lymphocytic-leukemia-cells-B-CLL-,10,2222,1,1.html