%0 Journal Article %J Archives of Medical Science %@ 1734-1922 %V 3 %N 1 %D 2007 %F Ołdak2007 %T Original paperArachidonic acid metabolism in TNF, CD178 (CD95 ligand) and ceramide-mediated cytotoxicity %X Introduction: Arachidonic acid release has been reported to play a significant role in TNF-induced cytotoxicity. However, the role of this signalling cascade in CD178-(CD95 ligand)mediated cytotoxicity remains unclear. The aim of the study was to investigate the role of arachidonic acid metabolism in TNF- and CD95-mediated cytotoxicity. Material and methods: Naturally TNF-sensitive A9 and CD178-sensitive A9-CD95 (the cells stably transfected with human CD95) cell lines were subjected to inhibitors of phospholipase A2 (AACOCF3, PACOCF3), lipoxygenase (NDGA) and cycloxygenase (indomethacin). Results: We showed that the cytotoxic activity of TNF could by inhibited by AACOCF3 and PACOCF3, inhibitors of phospholipase A2 as well as by NDGA, displaying anti-lipoxygenase and anti-oxidant inhibitory activity. In contrast, none of the tested inhibitors of arachidonic acid metabolism affected CD178-mediated cytotoxicity in A9-CD95 cells stably expressing CD95. Cytotoxicity mediated by C6-ceramide, a possible mediator of TNF- and CD178-mediated cytotoxicity, was also unaffected by these inhibitors. Conclusions: The above facts strongly suggest that CD95- as well as C6 ceramide-induced cell death does not depend on arachidonic acid metabolism. %A Ołdak, Monika %A Joźwiak, Jarosław %A Maksym, Radosław %A Malejczyk, Jacek %P 27-30 %9 journal article %U https://www.termedia.pl/Original-paper-Arachidonic-acid-metabolism-in-TNF-CD178-CD95-ligand-and-ceramide-mediated-cytotoxicity,19,7852,1,1.html