TY - JOUR JO - Central European Journal of Immunology SN - 1426-3912 VL - 35 IS - 3 PY - 2010 ID - Chorostowska-Wynimko2010 TI - Experimental immunologyDifferential regulation of tumor necrosis factor a and transforming growth factor β production by the plasminogen activator inhibitor 1 in endothelial and cancer cells AB - The plasminogen activator inhibitor (PAI-1) is the key component of fibrynolytic system, its synthesis is rapidly activated by cytokines and mediators driving inflammatory response, both acute and persistent low-grade. While tumor necrosis factor a (TNF-α) and transforming growth factor β (TGF-b) are established potent direct stimulators of PAI-1 production in numerous tissues, little is known of any feed-back regulation that might be accountable for effects of PAI-1 on their synthesis. Present study provides evidence for the existence of negative feed-back regulation between PAI-1 and its potent activator TGF-b in endothelial cells. Dose-dependent inhibition of TGF-b production was observed in HUVEC cultures with physiological (10 mg/ml) and supraphysiological (100 mg/ml) (p < 0.02) PAI-1 concentrations exerting significant suppressive effect in comparison to control (respectively p < 0.05; p < 0.02) as well as cultures spiked with subphysiological PAI-1 dose of 1 mg/ml (p < 0.0001; p < 0.02). No such regulation was demonstrated for cancer cells of lung and prostate origin which might implicate different regulatory mechanism in neoplastic cells. Similarly, no functional interplay between PAI-1 and TNF-α levels in 24 hrs cultures of any evaluated cell lines was observed. AU - Chorostowska-Wynimko, Joanna AU - Kędzior, Marta AU - Struniawski, Radosław AU - Gruber, Beata AU - Anuszewska, Elżbieta AU - Skrzypczak-Jankun, Ewa AU - Jankun, Jerzy DA - 2010 UR - https://www.termedia.pl/Experimental-immunology-Differential-regulation-of-tumor-necrosis-factor-a-and-transforming-growth-factor-production-by-the-plasminogen-activator-inhibitor-1-in-endothelial-and-cancer-cells,10,15406,1,1.html ER -