TY - JOUR JO - BioTechnologia SN - 0860-7796 VL - 92 IS - 2 PY - 2011 ID - Furmanek2011 TI - RESEARCH PAPEREmbryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings AB - The study investigates the regeneration capacity of Crambe abyssinica via the callus phase. The conducted experiments provide an overview of various conditions appropriate for callus development and its subsequent differentiation. Explants from cotyledons and leaves of sterile 14-day-old seedlings of C. abyssinica cv. Mayer were used. The primary callus so obtained was subsequently utilised for the observation of embryogenesis. The best callus formation on the explants was achieved on the agar-solidified MS medium with 0.5 mg ·dm -3 NAA and 0.5 mg ·dm -3 BAP or TDZ. The formation of secondary embryogenic callus was most efficient on MS medium supplemented with 0.4 mg ·dm -3 NAA and 0.3 mg ·dm -3 of BAP. AU - Furmanek, Tomasz AU - Banaś, Walentyna SP - 209 EP - 213 DA - 2011 DO - 10.5114/bta.2011.46535 UR - http://dx.doi.org/10.5114/bta.2011.46535 ER -