Abstract
4/2003
vol. 20
Basic microscopy, calculating the field of view, scanning of slides, sources of error
Post Derm Alerg 2003; XX, 4: 227–229
Online publish date: 2004/03/24
Airborne pollen samples are examined under a light microscope, by means of 250x or 400x magnification. To calculate the whole sample surface, the diameter of the microscopic field has to be given. There are four basic calculation methods. An investigator has to bear in mind possible sources of error, which can be associated with the reading method, sample interpretation and a preparation of a slide.
Keywords
light microscopy, pollens, field of view
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