Abstract
3/2013
vol. 17
Original paper
Expression of β-catenin and AXIN2 in ameloblastomas
Wspolczesna Onkol 2013; 17 (3): 250–256
Online publish date: 2013/06/28
Aim of the study: To investigate the expression status and association of β-catenin and AXIN2 in ameloblastoma.
Material and methods: 30 ameloblastoma specimens and 10 normal oral mucosa tissues were enrolled in the study. The protein and RNA levels of
β-catenin and AXIN2 were detected by immunohistochemistry staining, Western blot, and real-time PCR analysis. The relationship between β-catenin and AXIN2 protein and clinico-pathological parameters and prognosis was subsequently determined.
Results: The results show that β-catenin mRNA expression was 1.24-fold higher in ameloblastomas than that in normal mucosa tissues, but AXIN2 mRNA expression was 0.47-fold lower in ameloblastomas than that in normal mucosa tissues (p < 0.05). The Western blot results show that β-catenin and AXIN2 protein in ameloblastomas had a significantly higher level than normal mucosa tissues
(p < 0.05). Immunohistochemical staining of β-catenin and AXIN2 protein in ameloblastoma was expressed at a higher level than normal oral mucosa (p < 0.05).
Conclusions: AXIN2 and β-catenin play an important role in the tumorigenesis and progression of ameloblastoma.
Material and methods: 30 ameloblastoma specimens and 10 normal oral mucosa tissues were enrolled in the study. The protein and RNA levels of
β-catenin and AXIN2 were detected by immunohistochemistry staining, Western blot, and real-time PCR analysis. The relationship between β-catenin and AXIN2 protein and clinico-pathological parameters and prognosis was subsequently determined.
Results: The results show that β-catenin mRNA expression was 1.24-fold higher in ameloblastomas than that in normal mucosa tissues, but AXIN2 mRNA expression was 0.47-fold lower in ameloblastomas than that in normal mucosa tissues (p < 0.05). The Western blot results show that β-catenin and AXIN2 protein in ameloblastomas had a significantly higher level than normal mucosa tissues
(p < 0.05). Immunohistochemical staining of β-catenin and AXIN2 protein in ameloblastoma was expressed at a higher level than normal oral mucosa (p < 0.05).
Conclusions: AXIN2 and β-catenin play an important role in the tumorigenesis and progression of ameloblastoma.
Keywords
ameloblastoma, β-catenin, AXIN2, Wnt pathway
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