eISSN: 2083-8441
ISSN: 2081-237X
Pediatric Endocrinology Diabetes and Metabolism
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4/2020
vol. 26
 
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abstract:
Original paper

Metformin and proliferation of cancer cell lines

Karolina Tądel
1
,
Benita Wiatrak
2
,
Dorota Bodetko
2
,
Ewa Barg
2

1.
Faculty of Pharmacy, Wroclaw Medical University, Poland
2.
Department of Basic Medical Sciences, Wroclaw Medical University, Poland
Pediatr Endocrinol Diabetes Metab 2020; 26 (4): 159–166
Online publish date: 2020/11/13
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Introduction
Metformin is a widely used drug in treating type 2 diabetes and insulin resistance and nowadays scientists are searching for new poten-tial and multiple roles in prevention and treatment of carcinogenic processes.

Aim of the study
The aim of the study was to compare the impact of normoglycemia and hyperglycemia with doses of metformin on vivacity and prolifer-ation of cancer cell lines (MCF-7, MCF-7/DX, A549, CCRF/CEM, THP-1, NHDF).

Material and methods
We designed our experiment using raising glucose environment (40 mM, 100 mM, 150 mM, 300 mM) and we added increasing con-centrations of metformin (5 mM, 10 mM, 20 mM, 30 mM). We incubated cells for 24 h, 48 h, 72 h, 96 h. In order to measure of viabil-ity of cancer cells we use MTT assay – a typical test to mark cytotoxic effects of tested substances.

Results
Analysis indicated that populations of cancer cells in our terms was lowering, the incubation of 24 h and 48 h showed favorable results than 72 h and 96 h. In normoglycemic environment (glucose level about 100 mM) and after added metformin in various concentrations we observed decreasing percentage of vivid cells for all cancer cell lines (MCF-7, MCF-7/DX, A549, CCRF/CEM, THP-1).

Conclusions
The results of our study showed beneficial effects of metformin on decreasing proliferation of cancer cells. Percentage of vivid popula-tions were lowering and we confirmed anti-cancer effect of this drug.

keywords:

cancer cell lines, cell culture, growth, metformin, proliferation

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