The investigation was aimed to evaluate activity of the cancer procoagulant in blood serum and cancer tissue of patients with renal cancer and its use in the oncological urology diagnostics. Twenty three patients with renal cancer made up the investigated group (serum and cancer tissue). The control group consisted of serum of the 15 healthy individuals and the normal renal tissue localized close to the renal cancer. The CP activity expressed by the coagulation time (in seconds) has been determined according to the Gordon and Benson’s coagulation method.
The CP activity in 10% tissue homogenate has been determined by means of spectrophotometry using the Colluci chromogen method where the enzyme activity is expressed by nmol of released p-nitroanilin (pNa/mL).
The CP activity in patients’ serum reached 98.5±23.0 s for kidney cancer patients and 293.7±21.4 s in healthy persons.
The CP activity in 10% tissue homogenate of the renal cancer was 40.8±14.6 nmol of pNa/mL and in the normal tissue reached 23.8±9.3 nmol of pNa/mL.
The said difference is significant statistically.
That is why we suggest using the idea of CP activity determination in the renal cancer diagnostics.