Journal of Stomatology

Abstract

4/2025 vol. 78
Original paper

Comparative evaluation of pH, and calcium and fluoride ion release between two pulp capping agents at different time periods: an in vitro study

Shreya Gokul Shirsath 1
,
Karan Bhargava 1, 2
,
Srindhi Surya Raghavendra 1
,
Sanjyot Mulay 1
,
Apeksha Gambhir 1
,
Riddhi Kakodkar 1
  1. Department of Conservative Dentistry and Endodontics, Dr. D.Y. Patil Dental College and Hospital, Dr. D.Y. Patil Vidyapeeth, Pune, India
  2. School of Dentistry and Oral Health, College of Medicine, Nursing and Health Sciences Fiji National University, Suva, Fiji
J Stoma 2025; 78, 4: 245-249
DOI: https://doi.org/10.5114/jos.2025.155740
Online publish date: 2025/11/03
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Introduction

The success of dental pulp capping procedures relies heavily on the selection of materials with optimal biological and physicochemical properties.

Objectives

To evaluate and compare pH, and calcium and fluoride ion release of TheraBase and Biner LC at 2 hours, 24 hours, 48 hours, 7 days, and 21 days time intervals.

Material and methods

Thirty cylindrical samples were prepared and suspended in 10 ml deionized water. They were divided into 2 groups (TheraBase, n = 15; and Biner LC, n = 15), and further divided into 3 subgroups according to parameters. They were stored for 2 hours, 24 hours, 48 hours, 7 days, and 21 days. Solution used for storage was analyzed for measurement of all three parameters (pH, calcium and fluoride ion release) at each time period. Fresh 10 ml deionized water in a new container was employed to suspend the samples after drying, and storage was continued till the next time period. EDTA titration method was used to confirm calcium ion release. SPADNS spectrophotometric method was applied to check fluoride ion release, while pH was examined with a pH meter.

Results

pH, and calcium and fluoride ion release were consistently higher in group 1 (TheraBase) than group 2 (Biner LC). Also, group 1 showed a gradual increase of calcium ion release with time, while fluoride release and pH were consistent at all time periods for both the groups (p < 0.05).

Conclusions

TheraBase proved to have a higher pH and ion release than Biner LC. However, both the mate­rials showed a sustained release of calcium and fluoride ions, rendering them bioactive pulp capping agents.

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Keywords

pH, ion release, bioactive material, pulp capping agent

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