Abstract
4/2025
vol. 29
Original paper
Cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC fruit on B16F10 melanoma cell culture
- Department of Physiology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
- Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia
- Department of Dermatology and Venerology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
- Philosophy Doctor in Medicine Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia
Contemp Oncol (Pozn) 2025; 29 (4): 354–359
Online publish date: 2025/11/03
This study aimed to investigate the cytotoxicity and apoptotic effects of the ethyl acetate fraction of Zanthoxylum acanthopodium DC (ZA) fruit on B16F10 melanoma cell cultures.
Material and methods: The ethyl acetate fraction was obtained through maceration followed by fractionation with n-hexane and ethyl acetate. Cytotoxicity was assessed using the MTT assay to determine the IC50 value. B16F10 melanoma cells were then grouped into control, IC50, ¾ IC50, ½ IC50, and doxorubicin groups. Apoptosis induction was analysed using Annexin V and caspase-3 expression assays.
Results: The ethyl acetate fraction of ZA fruit exhibited cytotoxic activity on B16F10 melanoma cells, with an IC50 value of 249.3 µg/ml. Apoptosis induction was confirmed by a significant increase in caspase-3 expression compared to the control group (p < 0.05).
Conclusions: The ethyl acetate fraction of ZA fruit demonstrated cytotoxic effects and induced apoptosis in B16F10 melanoma cells, potentially through caspase-3 activation.
Material and methods: The ethyl acetate fraction was obtained through maceration followed by fractionation with n-hexane and ethyl acetate. Cytotoxicity was assessed using the MTT assay to determine the IC50 value. B16F10 melanoma cells were then grouped into control, IC50, ¾ IC50, ½ IC50, and doxorubicin groups. Apoptosis induction was analysed using Annexin V and caspase-3 expression assays.
Results: The ethyl acetate fraction of ZA fruit exhibited cytotoxic activity on B16F10 melanoma cells, with an IC50 value of 249.3 µg/ml. Apoptosis induction was confirmed by a significant increase in caspase-3 expression compared to the control group (p < 0.05).
Conclusions: The ethyl acetate fraction of ZA fruit demonstrated cytotoxic effects and induced apoptosis in B16F10 melanoma cells, potentially through caspase-3 activation.
Keywords
melanoma, apoptosis, B16F10, ethyl acetate fraction, Zanthoxylum acanthopodium DC
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