Effect of bromelain and trypsin on microleakage of etch-and-rinse adhesive systems

Introduction
The removal of collagen by chemical methods can effectively decrease the marginal microleakage of composites used in restorative dentistry.

Objectives
To present research focused on the assessment of the impact of surface treatment of dentin with 5% bromelain and 5% trypsin on the microleakage of composites. To present research focused on the assessment of the impact of surface treatment of dentin with 5% bromelain and 5% trypsin on the microleakage of composites.

Material and methods
In the lingual and buccal surfaces of 60 extracted premolars, class V cavities were prepared in standard condition. The test cases were divided into three groups (n = 40). In the control group, 37% phosphoric acid was employed for etching the cavities. In one of the groups, Adper Single Bond was utilised in the wall of cavity, while OptiBond Solo was applied in the other group. Afterwards, the samples were restored by employing Z250 composite. 5% bromelain and 5% trypsin were used in the test groups. The samples were then immersed in 2% fuchsine solution to analyse the marginal microleakage.

Results
No significant difference was observed in the occlusal marginal microleakage of study groups (p = 0.43), but the difference in microleakage of cervical margins was significant (p = 0.000).

Conclusions
Pretreatment of cavity by using 5% bromelain in the etched dentin surface showed no significant impact on microleakage. However, using 5% trypsin after etching has a negative effect on the microleakage at the cervical margins.