eISSN: 1897-4309
ISSN: 1428-2526
Contemporary Oncology/Współczesna Onkologia
Current issue Archive Manuscripts accepted About the journal Supplements Addendum Special Issues Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
6/2009
vol. 13
 
Share:
Share:
abstract:
Case report

Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature

Jan Styczyński
,
Małgorzata Rochnowska
,
Robert Dębski
,
Elżbieta Hulek
,
Anna Krenska
,
Hanna Górnicka
,
Paweł Wojtylak
,
Mariusz Wysocki

Współczesna Onkologia (2009) vol. 13; 6 (292–296)
Online publish date: 2010/01/04
View full text Get citation
 
A haploidentical transplant can be defined as a transplant that uses marrow or stem cells from a relative who is only half matched in HLA. Recently, a new regimen using graft CD3/CD19 depletion with anti-CD3- and anti-CD19-coated microbeads on a CliniMACS device was developed. CD3/CD19 depleted grafts contain not only CD34+ stem cells but also CD34-negative progenitors, natural killer, dendritic and graft-facilitating cells, thereby enhancing engraftment. We report a case description of a child using CD3/CD19 depleted grafts in a haploidentical stem cell transplant setting and a review of the available literature. A 3-year old boy was treated for very early relapse of acute lymphoblastic leukaemia. The myelo-ablative therapy consisted of weight-adjusted intravenous busulfan, flu-darabine, cyclophosphamide, and thymoglobuline. The reduction of CD3 load was 99.9% (i.e. 3.64 log scale); however, due to current CD3 load in the graft (2.7 × 105 CD3+/kg), GVHD prophylaxis was done with cyclosporine A and methotrexate. The only complication after transplantation was CMV reactivation, treated with pre-emptive therapy. Bone marrow biopsy at day +29 revealed normal haematopoiesis and donor chimerism 95%. The patient remains in remission at day +300. We can conclude from our observation and literature review that HLA haploidentical peripheral blood stem cell transplantation with CD3/CD19 depletion may be an effective and safe therapy for children with relapsed leukaemia.
keywords:

stem cell transplantation, children, CD3/CD19 depletion, acute lymphoblastic leukaemia

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.