High expression of the eukaryotic initiation factors eIF4a and eIF4e in retinoblastoma

Nora Fischer; Antje Schinlauer; Piotr Czapiewski; Yvonne Garbers; Christoph Garbers

doi:10.5114/wo.2025.153882

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

Current issue Archive Manuscripts accepted About the journal Supplements Addendum Special Issues Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

3/2025
vol. 29

Send email

Copy url:

abstract:

Original paper

High expression of the eukaryotic initiation factors eIF4a and eIF4e in retinoblastoma

Nora Fischer

¹

,

Antje Schinlauer

¹

,

Piotr Czapiewski

^{1, 2}

,

Yvonne Garbers

³

,

Christoph Garbers

⁴

Department of Pathology, Otto-von-Guericke-University Magdeburg, 39120 Magdeburg, Germany
Department of Pathology, Staedtisches Klinikum Dessau, Brandenburg Medical School Theodor Fontane and Faculty of Health Sciences Brandenburg, Dessau, Germany
Faculty of Management, Culture and Technology (Lingen campus), Osnabrück University of Applied Sciences, 49809 Lingen, Germany
Institute of Clinical Biochemistry, Hannover Medical School, 30625 Hannover, Germany

Contemp Oncol (Pozn) 2025; 29 (3): 257–266

DOI: https://doi.org/10.5114/wo.2025.153882

Online publish date: 2025/09/17

View full text Get citation

PlumX metrics:

Introduction:
Retinoblastoma is a malignant, intraocular tumor which develops within the first three years of life and has a variable prognosis. Aberrant expression of eukaryotic initiation factors (eIFs) has been reported in a variety of tumor entities, but there is a lack of data regarding eIF expression in retinoblastoma.

Material and methods:
We analyzed the expression of eIF4a and eIF4e in 30 retinoblastomas and samples of other ocular diseases via immunohistochemistry. Via western blot, we determined the expression of eIF4a and eIF4e in the two retinoblastoma cell lines WERI-Rb1 and Y79. Furthermore, we evaluated the influence of the eIF inhibitor 4EGI-1 on the cell viability of both cancer cell lines.

Results:
In this study, we observed increased expression of eIF4a and eIF4e in retinoblastoma samples compared to other ocular diseases. We also found that both proteins are expressed in the retinoblastoma cell lines WERI-Rb1 and Y79 and that their expression is independent of treatment with the eIF inhibitor 4EGI-1. However, inhibition of eIF function via 4EGI-1 reduced the cell viability of both cell lines in a dose- and time-dependent manner.

Conclusions:
We provide evidence that eIF4a and eIF4e are overexpressed in retinoblastoma and that their inhibition might represent a therapeutic target for the therapy of retinoblastoma.

keywords:

High expression of the eukaryotic initiation factors eIF4a and eIF4e in retinoblastoma

Nora Fischer 1 , Antje Schinlauer 1 , Piotr Czapiewski 1, 2 , Yvonne Garbers 3 , Christoph Garbers 4

eIFs, 4EGI-1, retinoblastoma cells

Nora Fischer

¹

,

Antje Schinlauer

¹

,

Piotr Czapiewski

^{1, 2}

,

Yvonne Garbers

³

,

Christoph Garbers

⁴