Modulation of hormones in perimenopausal women by probiotics associated by specific gut bacteria: a pilot study
Division of Health Sciences, Anáhuac University Querétaro, Circuito Universidades I, Querétaro, Mexico
Facultad de Ciencias Naturales, Universidad Autónoma de Querétaro, Querétaro, Mexico
Centro de Investigación Biomédica Avanzada, Facultad de Medicina, Universidad Autónoma de Querétaro, Mexico
Menopause Rev 2026; 25(2)
Data publikacji online: 2026/08/23
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- Harlow SD, Gass M, Hall JE, Lobo R, Maki P, Rebar RW, et al. Executive summary of the stages of reproductive aging workshop + 10: addressing the unfinished agenda of staging reproductive aging. Menopause 2012; 19 : 387-395.
- Chen W, Chen M, Tang H, Wei W, Shao P, Dou S, et al. Advances in diagnosis and treatment of perimenopausal syndrome. Open Life Sci 2023; 18: 20220754.
- Duralde ER, Sobel TH, Manson JE. Management of perimenopausal and menopausal symptoms. BMJ 2023; 382: e072612.
- Li JY, Chassaing B, Tyagi AM, Vaccaro C, Luo T, Adams J, et al. Sex steroid deficiency-associated bone loss is microbiota dependent and prevented by probiotics. J Clin Invest 2016; 126: 2049-2063.
- Lee J, Peesh P, Quaicoe V, Tan C, Banerjee A, Mooz P, et al. Estradiol mediates colonic epithelial protection in aged mice after stroke and is associated with shifts in the gut microbiome. Gut Microbes 2023; 15: 2271629.
- Filippone A, Rossi C, Rossi MM, Di Micco A, Maggiore C, Forcina L, et al. Endocrine disruptors in food, estrobolome and breast cancer. J Clin Med 2023; 12: 3158.
- Pai AHY, Wang YW, Lu PC, Wu HM, Xu JL, Huang HY. Gut microbiome-estrobolome profile in reproductive-age women with endometriosis. Int J Mol Sci 2023; 24: 16301.
- Baker JM, Al-Nakkash L, Herbst-Kralovetz MM. Estrogen-gut microbiome axis: physiological and clinical implications. Maturitas 2017; 103: 45-53.
- Flores R, Shi J, Fuhrman B, Xu X, Veenstra TD, Gail MH, et al. Fecal microbial determinants of fecal and systemic estrogens and estrogen metabolites: a cross-sectional study. J Transl Med 2012; 10: 253.
- Peters BA, Santoro N, Kaplan RC, Qi Q. Spotlight on the gut microbiome in menopause: current insights. Int J Womens Health 2022; 14: 1059-1072.
- Samavat H, Kurzer MS. Estrogen metabolism and breast cancer. Cancer Lett 2015; 356 : 231-243.
- Boutriq S, González-González A, Plaza-Andrades I, Laborda-Illanes A, Sánchez-Alcoholado L, Peralta-Linero J, et al. Gut and endometrial microbiome dysbiosis: a new emergent risk factor for endometrial cancer. J Pers Med 2021; 11: 659.
- Carnevali O, Avella MA, Gioacchini G. Effects of probiotic administration on zebrafish development and reproduction. Gen Comp Endocrinol 2013; 188: 297-302.
- Nakatsu CH, Armstrong A, Clavijo AP, Martin BR, Barnes S, Weaver CM. Fecal bacterial community changes associated with isoflavone metabolites in postmenopausal women after soy bar consumption. PLoS One 2014; 9: e108924.
- Kadry MO, Megeed RA. Probiotics as a complementary therapy in the model of cadmium chloride toxicity: crosstalk of -catenin, BDNF, and StAR signaling pathways. Biol Trace Elem Res 2018; 185: 404-413.
- Hu S, Ding Q, Zhang W, Kang M, Ma J, Zhao L. Gut microbial beta-glucuronidase: a vital regulator in female estrogen metabolism. Gut Microbes 2023; 15: 2236749.
- Vicariotto F, Malfa P, Viciani E, Dell’Atti F, Squarzanti DF, Marcante A, et al. Efficacy of Lactiplantibacillus Plantarum PBS067, Bifidobacterium Animalis Subsp. Lactis BL050, and Lacticaseibacillus Rhamnosus LRH020 in the amelioration of vaginal microbiota in post-menopausal women: a prospective observational clinical trial. Nutrients 2024; 16: 402.
- Lim EY, Lee SY, Shin HS, Lee J, Nam YD, Lee DO, et al. The effect of lactobacillus acidophilus YT1 (MENOLACTO) on improving menopausal symptoms: a randomized, double-blinded, placebo-controlled clinical trial. J Clin Med 2020; 9: 2173.
- Boyle RJ, Robins-Browne RM, Tang MLK. Probiotic use in clinical practice: what are the risks? Am J Clin Nutr 2006; 83: 1256-1264.
- Hijová E, Kuzma J, Strojný L, Bomba A, Bertková I, Chmelárová A, et al. Ability of Lactobacillus Plantarum LS/07 to modify intestinal enzymes activity in chronic diseases prevention. Acta Biochim Pol 2017; 64: 113-116.
- Wang M, Ma H, Shi Y, Ni H, Qin C, Ji C. Single-arm clinical trials: design, ethics, principles. BMJ Supp Palliat Care 2024; 15: 46-54.
- Travers C, O’Neill SM, King R, Battistutta D, Khoo SK. Greene climacteric scale: norms in an Australian population in relation to age and menopausal status. Climacteric 2005; 8: 56-62.
- Rinttilä T, Kassinen A, Malinen E, Krogius L, Palva A. Development of an extensive set of 16S rDNA-targeted primers for quantification of pathogenic and indigenous bacteria in faecal samples by real-time PCR. J Appl Microbiol 2004; 97: 1166-1177.
- Collado MC, Isolauri E, Laitinen K, Salminen S. Distinct composition of gut microbiota during pregnancy in overweight and normal-weight women. Am J Clin Nutr 2008; 88: 894-899.
- Guo X, Xia X, Tang R, Zhou J, Zhao H, Wang K. Development of a real-time PCR method for firmicutes and bacteroidetes in faeces and its application to quantify intestinal population of obese and lean pigs. Lett Appl Microbiol 2008; 47: 367-373.
- Hatakka K, Mutanen M, Holma R, Saxelin M, Korpela R. Lactobacillus Rhamnosus LC705 together with propionibacterium Freudenreichii Ssp Shermanii JS administered in capsules is ineffective in lowering serum lipids. J Am Coll Nutr 2008; 27: 441-447.
- Rerksuppaphol S, Rerksuppaphol L. A randomized double-blind controlled trial of Lactobacillus Acidophilus plus Bifidobacterium Bifidum versus placebo in patients with hypercholesterolemia. J Clin Diagn Res 2015; 9: KC01-04.
- El Khoudary SR, Chen X, Wang Z, Brooks MM, Orchard T, CrawfordS, et al. Low-density lipoprotein subclasses over the menopausal transition and risk of coronary calcification and carotid atherosclerosis: the SWAN heart and HDL ancillary studies. Menopause 2023; 30: 1006-1013.
- Bernini LJ, Simão ANC, Alfieri DF, Lozovoy MAB, Mari NL, de Souza CHB, et al. Beneficial effects of Bifidobacterium Lactis on lipid profile and cytokines in patients with metabolic syndrome: a randomized trial. Effects of probiotics on metabolic syndrome. Nutrition 2016; 32: 716-719.
- Tom Dieck H, Schön C, Wagner T, Pankoke HC, Fluegel M, Speckmann B. A synbiotic formulation comprising bacillus subtilis DSM 32315 and L-alanyl-L-glutamine improves intestinal butyrate levels and lipid metabolism in healthy humans. Nutrients 2021; 14: 143.
- Lye HS, Rusul G, Liong MT. Removal of cholesterol by lactobacilli via incorporation and conversion to coprostanol. J Dairy Sci 2010; 93: 1383-1392.
- Tsai CC, Lin PP, Hsieh YM, Zhang Z, Wu HC, Huang CC. Cholesterol-lowering potentials of lactic acid bacteria based on bile-salt hydrolase activity and effect of potent strains on cholesterol metabolism in vitro and in vivo. Sci World J 2014; 2014: 690752.
- Momin ES, Khan AA, Kashyap T, Pervaiz MA, Akram A, Mannan V, et al. O. The effects of probiotics on cholesterol levels in patients with metabolic syndrome: a systematic review. Cureus 2023; 15: e37567.
- Hall JE. Endocrinology of the menopause. Endocrinol Metab Clin North Am 2015; 44: 485-496.
- Zou S, Yang X, Li N, Wang H, Gui J, Li J. Association of probiotic ingestion with serum sex steroid hormones among pre- and postmenopausal women from the NHANES, 2013 – 2016. PLoS One 2023; 18: e0294436.
- Honda S, Tominaga Y, Espadaler-Mazo J, Huedo P, Aguiló M, Perez M, et al. Supplementation with a probiotic formula having -glucuronidase activity modulates serum estrogen levels in healthy peri- and postmenopausal women. J Med Food 2024; 27: 720-727.
- Cotton S, Clayton CA, Tropini C. Microbial endocrinology: the mechanisms by which the microbiota influences host sex steroids. Trends Microbiol 2023; 31: 1131-1142.
- Muccee F, Ghazanfar S, Ajmal W, Al-Zahrani M. In silico characterization of estrogen reactivating -glucuronidase enzyme in GIT associated microbiota of normal human and breast cancer patients. Genes (Basel) 2022; 13: 1545.
- Sui Y, Wu J, Chen J. The Role of gut microbial -glucuronidase in estrogen reactivation and breast cancer. Front Cell Dev Biol 2021; 9: 631552.
- Ervin SM, Li H, Lim L, Roberts LR, Liang X, ManiS, et al. Gut microbial -glucuronidases reactivate estrogens as components of the estrobolome that reactivate estrogens. J Biol Chem 2019; 294: 18586-18599.
- Walsh J, Olavarria-Ramirez L, Lach G, Boehme M, Dinan TG, Cryan JF, et al. Impact of host and environmental factors on -glucuronidase enzymatic activity: implications for gastrointestinal serotonin. Am J Physiol Gastrointest Liver Physiol 2020; 318: G816-G826.
- Gao S, Sun R, Singh R, Yu So S, Chan CTY, Savidge T, et al. The role of gut microbial -glucuronidase in drug disposition and development. Drug Discov Today 2022; 27: 103316.
- Mroczyńska M, Libudzisz Z. Beta-glucuronidase and beta-glucosidase activity of Lactobacillus and Enterococcus isolated from human feces. Pol J Microbiol 2010; 59: 265-269.
- Han S, Lu Y, Xie J, Fei Y, Zheng G, Wang Z, et al. Probiotic gastrointestinal transit and colonization after oral administration: a long journey. Front Cell Infect Microbiol 2021; 11: 609722.
- Santoro N, Roeca C, Peters BA, Neal-Perry G. The menopause transition: signs, symptoms, and management options. J Clin Endocrinol Metab 2021; 106: 1-15.
- Andrews R, Lacey A, Bache K, Kidd EJ. The role of menopausal symptoms on future health and longevity: a systematic scoping review of longitudinal evidence. Maturitas 2024; 190: 108130.
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