The aim of this study was wound healing in 43 women after vulvectomy because of vulvar squamosus cell carcinoma after surgery procedures in years 2003-2004, in departament of obstetrics and gynecology in Bytom. In 90.7% patients we observed pathological bacterial flora in swabs from vulva. We observed Escherichia coli, Streptococcus β haemoliticus gr. B, Escherichia coli haemoliticum , Staphylococcus aureus, Proteus mirabilis, Gardnerella vaginalis, Morganella morgani oraz Pseudomonas aeruginosa. Before operation all women with pathological bacterial flora were treated with antibiotics. In 62.8% women after surgery procedures we observed well wound healing and in 37.2% patients the process of wound healing was disturbed. In that group we observed Escherichia coli, Staphylococcus aureus, Proteus mirabilis, Streptococcus β haemoliticus gr. B, Morganella morgani oraz Pseudomonas aeruginosa.