Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Efe Koyun; Remzi Karadag; Seyma Ozkanli; Serpil Oguztuzun; Arzu Kaya Kocdogan; Isilay Ozsoy

doi:10.5114/ada.2020.98285

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

Current issue Archive Manuscripts accepted About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

4/2020
vol. 37

Send email

Copy url:

abstract:

Original paper

Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Efe Koyun

¹

,

Remzi Karadag

²

,

Seyma Ozkanli

³

,

Serpil Oguztuzun

⁴

,

Arzu Kaya Kocdogan

⁴

,

Isilay Ozsoy

¹

Department of Ophthalmology, Kastamonu State Hospital, Kastamonu, Turkey
Department of Ophthalmology, School of Medicine, Istanbul Medeniyet University, Goztepe, Istanbul, Turkey
Department of Pathology, School of Medicine, Istanbul Medeniyet University, Goztepe, Istanbul, Turkey
Department of Biology, School of Medicine, Kirikkale University, Kirikkale, Turkey

Adv Dermatol Allergol 2020; XXXVII (4): 535–539

DOI: https://doi.org/10.5114/ada.2020.98285

Online publish date: 2020/09/02

View full text Get citation

Introduction
Eyelid tumours mostly originated from skin and its appendeges. External carcinogens like UV radiation causes cell damages in the eyelid skin and contributes to carcinogenesis. Apoptosis is a very important mechanism to prevent these damage and probable neoplatic change.

Aim
To compare caspase-3, p53 and Bcl-2 levels between patients with basal cell carcinoma (BCC) of the eyelid and healthy individuals.

Material and methods
Pathology archives from October 2012 to April 2015 were scanned for BCC biopsies of the eyelid and tissue removed during blepharoplasty and entropion procedures. A total of 36 specimens were found. The specimens were divided into two groups: BCC group and controls (consisting of eyelid tissue removed during routine blepharoplasty). The pathology specimens were then stained using p53, Bcl-2 and caspase-3 stains and the intensity of staining was graded on a 0–3 scale.

Results
Samples from a total of 36 patients were included in the study. Eighteen (50.0%) patients were female. There were 13 patients in the BCC group and 23 patients in the control group. The mean age was 66.0 ±10.8 years in the BCC group, and 65.61 ±11.22 years in the control group. The caspase-3 staining was lower in the BCC group than in the control group. No significant differences were found between the BCC group and the control group in terms of p53 levels or Bcl-2 levels (both of them, p = 1.000).

Conclusions
The caspase-3 level was lower in the BCC group. This result suggests that these enzymes can play a significant role in carcinogenesis of eyelid BCC.

keywords:

Caspase-3, p53 and Bcl-2 expression in basal cell carcinoma of the eyelid

Efe Koyun 1 , Remzi Karadag 2 , Seyma Ozkanli 3 , Serpil Oguztuzun 4 , Arzu Kaya Kocdogan 4 , Isilay Ozsoy 1

basal cell carcinoma, Bcl-2, caspase-3, eyelid tumour, immunohistochemical stain, p53, malignant tumour

Efe Koyun

¹

,

Remzi Karadag

²

,

Seyma Ozkanli

³

,

Serpil Oguztuzun

⁴

,

Arzu Kaya Kocdogan

⁴

,

Isilay Ozsoy

¹