Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study

Małgorzata Guz; Agnieszka Mądro; Marek Cybulski; Witold Jeleniewicz; Joanna Kozicka; Jacek Kurzepa

doi:10.5114/ms.2023.129039

eISSN: 2300-6722
ISSN: 1899-1874

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abstract:

Original paper

Correlations of miR-1290 and miR-191-5p with laboratory parameters as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study

Małgorzata Guz

¹

,

Agnieszka Mądro

²

,

Marek Cybulski

¹

,

Witold Jeleniewicz

¹

,

Joanna Kozicka

²

,

Jacek Kurzepa

³

Department of Biochemistry and Molecular Biology, Medical University of Lublin, Lublin, Poland
Department of Gastroenterology with Endoscopic Unit, Medical University of Lublin, Lublin, Poland
Department of Medical Chemistry, Medical University of Lublin, Lublin, Poland

Medical Studies/Studia Medyczne 2023; 39 (2): 114–121

DOI: https://doi.org/10.5114/ms.2023.129039

Online publish date: 2023/06/30

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AMA

Guz M, Mądro A, Cybulski M, Jeleniewicz W, Kozicka J, Kurzepa J. Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study. Medical Studies/Studia Medyczne. 2023;39(2):114-121. doi:10.5114/ms.2023.129039.

APA

Guz, M., Mądro, A., Cybulski, M., Jeleniewicz, W., Kozicka, J.,  & Kurzepa, J. (2023). Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study. Medical Studies/Studia Medyczne, 39(2), 114-121. https://doi.org/10.5114/ms.2023.129039

Chicago

Guz, Małgorzata, Agnieszka Mądro, Marek Cybulski, Witold Jeleniewicz, Joanna Kozicka,  and Jacek Kurzepa. 2023. "Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study". Medical Studies/Studia Medyczne 39 (2): 114-121. doi:10.5114/ms.2023.129039.

Harvard

Guz, M., Mądro, A., Cybulski, M., Jeleniewicz, W., Kozicka, J.,  and Kurzepa, J. (2023). Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study. Medical Studies/Studia Medyczne, 39(2), pp.114-121. https://doi.org/10.5114/ms.2023.129039

MLA

Guz, Małgorzata et al. "Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study." Medical Studies/Studia Medyczne, vol. 39, no. 2, 2023, pp. 114-121. doi:10.5114/ms.2023.129039.

Vancouver

Guz M, Mądro A, Cybulski M, Jeleniewicz W, Kozicka J, Kurzepa J. Correlations of miR-1290 and miR-191-5p with laboratory parameters
as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study. Medical Studies/Studia Medyczne. 2023;39(2):114-121. doi:10.5114/ms.2023.129039.

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Introduction
Accurate differential diagnosis between chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC) is still problematic. Difficulties arise from the fact that the imaging techniques, clinical symptoms, and laboratory parameters of these 2 diseases are similar. Therefore, non-invasive markers to clearly distinguish between these 2 disorders are urgently needed, and microRNAs (miRNAs) seem to be the most promising biomarkers, because of their tissue specificity, stability in different biofluids, and easy detection.

Aim of the research
To evaluate the usefulness of the measurement of selected miRNAs and basic clinical parameters as a test for differentiating PDAC from CP.

Material and methods
The expression of miR-21-5p, miR-23a-3p, miR-155-5p, miR-191-5p, miR-196a-5p, miR-205-5p, and miR-1290 was assessed in 74 serum samples (PDAC n = 26, CP n = 34, and the control group n = 14) by reverse transcription-quantitative PCR (RT-qPCR). Correlations between the levels of miRNAs and pancreatic lipase, amylase, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), g-glutamyltranspeptidase (GGTP), haemoglobin (Hb), bilirubin, C-reactive protein (CRP), and CA19-9 were analysed in 3 groups.

Results
We found that 2 combinations of miRNAs with blood parameters had diagnostic value. In PDAC the expression of miR-1290 positively correlated with GGTP activity (p = 0.029, rs = 0.487) and CA 19-9 level (p = 0.026, rs = 0.464), while in CP patients miR-191-5p expression was negatively correlated with amylase (p = 0.018, rs = –0.408) and CRP (p = 0.023, rs = –0.394). No statistically significant correlations were noticed between miRNAs and clinical parameters in the control group.

Conclusions
The combination of miR-191-5p with CRP and amylase characterized CP, while miR-1290 with GGTP and CA 19-9 was specific for PDAC. These non-invasive tools can help to differentiate PDAC from CP.

keywords:

Correlations of miR-1290 and miR-191-5p with laboratory parameters as a useful test to differentiate pancreatic cancer from chronic pancreatitis: preliminary study

Małgorzata Guz 1 , Agnieszka Mądro 2 , Marek Cybulski 1 , Witold Jeleniewicz 1 , Joanna Kozicka 2 , Jacek Kurzepa 3

differential diagnosis, miRNAs, pancreatitis, pancreatic ductal adenocarcinoma

Małgorzata Guz

¹

,

Agnieszka Mądro

²

,

Marek Cybulski

¹

,

Witold Jeleniewicz

¹

,

Joanna Kozicka

²

,

Jacek Kurzepa

³