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Journal of Stomatology
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Original paper

Effects of different impression methods and holding times on the dimensional accuracy of addition silicones

Shiva Mahboubi
1
,
Behnaz Mollai
2
,
Mahdi Rahbar
3

  1. Prosthodontics Department, Dentistry Faculty, Kurdistan University of Medical Sciences, Sanandaj, Iran
  2. Private Dentist Practitioner, Hamadan, Iran
  3. Restorative and Esthetic Dentistry Department, Dentistry Faculty, Tabriz University of Medical Sciences, Tabriz, Iran
J Stoma 2020; 73, 1: 15-21
Online publish date: 2020/04/08
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- JoS_00055_Mahboubi.pdf  [1.00 MB]
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INTRODUCTION

An impression is the negative form or shape of the original form. The impression is created by placing soft and semi-fluid materials in the mouth and restoring it after being set. Depending on the materials used, the impression may be hard or elastic. Materials that are usually used for casting restorations should be elastic, when taken out of the mouth. Since the restoration must be accurate, the teeth cast from the impression must also be accurate, with no difference. The impression should also be stored carefully before casting. In many cases, the impression shape is changed due to improper maintenance or unnecessary delays between the impression and casting [1].
Despite the advancements in CAD/CAM and the 3D imaging, conventional impression is still widely used. Various factors are effective in the preparation of an accurate impression. Specifically, the conditions of the periodontal membrane of teeth, oral hygiene, location of the finishing line, impression technique, trays used, and precision of the impression material are highly influential [2].
An ideal impression material should not be so hard, so that it can be easily removed from the undercuts. It should also have an elastic recovery property to allow the material to return to its original dimensions once withdrawn from the dental undercuts and prevent it from undergoing a permanent change [3]. It must also have sufficient strength when taken out of the mouth to prevent rupture [4]. Today, there are many types of impression materials, which seem to be reasonably accurate [1]. According to numerous studies, addition silicon or polyvinyl siloxanes are one of the most accurate impression materials in the manufacture of fixed restorations. This material, along with impression materials, has the least variations in casting or recasting [5].
As mentioned above, the impression technique plays a decisive role in the quality of impressions [1]. If the impression methodology is not sufficiently accurate, the impression will undergo dimensional changes, and it will not match correctly at the end of restoration [6]. Common techniques in fixed prosthodontic impression are one- and two-stage methods. In the one-stage method, putty and wash are used simultaneously [7]. The disadvantage of this technique is lack of control over the thickness of the wash [8]. In response, the two-stage impression method is used to overcome the problem of controlling the thickness of wash. In this method, first, the impression is performed by putty, after which, a wash is used in the second stage to record the details [7]. According to ADA no.19, the space provided for the wash material can affect the impression accuracy. If the wash material cannot move freely through the pores of the putty, in the second stage of impressioning, the wash compresses the putty and causes its permanent deformation and irregularities in the impression [4].
Sometimes impressions are send to a laboratory technician at a later time; however, in this case, there is concern about the dimensional variation of impression materials over time [3]. So, this time limit is very important.

OBJECTIVES

Considering the contradictions in studies, the current paper aims to investigate the effect of different impression methods on the dimensional accuracy of casts and different holding times on the dimensional accuracy of addition silicones. Another purpose of the study was choosing the best method for impression and the best impression time, so that the final restorations have the highest accuracy.

MATERIAL AND METHODS

The sample size was calculated to be 12 samples in each subgroup, and a total of 144 samples were included with confidence level of 95% and test power of 90%, as per Sazegara et al. study [7] (taking into account minimum mean of 7.31, maximum of 7.37, and standard deviation of 0.04). The 144 samples were prepared to study the effect of different impression methods and holding times on the dimensional accuracy. For each of the main impression groups, 48 samples were prepared based on the impression technique, and these samples were divided into 4 subgroups (each consisting of 12 samples) based on the holding time.

METAL MODEL DEVELOPMENT

In order to study the dimensional accuracy, the utilized model was in the form of two metal pyramid dies, one with occlusal diameter of 9.39 mm and height of 12.84 mm, and the other with occlusal diameter of 12.84 mm and height of 12.80 mm. Each one was placed on a metal pillar with a convergence angle of 6º, 44.20 mm away from the other (similar to the pillars of a long bridge). Each had a shoulder finishing line of 1 mm (Figure 1). The obtained measurements in the gypsum samples included diameter and height of smaller die with the two dies distance from at the occlusal part. Finally, the measurements were compared with the main model.

MAKING 5-MM SPACER

In order to maintain the necessary space for the uniform material and the uniform thickness of the putty at all points, a 5-mm spacer was made with a green acryl (Acropars, Tehran, Iran). A caliper was used to measure the uniform thickness. Equal-sized holes were made in the four sides of the spacer, so that when making the acryl tray, the acryl penetrates into the holes and create a stop.

MAKING ACRYLIC SPECIAL TRAY

To make a special tray, first the 5-mm wax spacer was placed on a metal model and 12 trays were made from green acryl. Tray edges were 10 mm lower than the hori¬zontal floor of metal model to get enough thickness in the gypsum of samples.

CREATING SPACER FOR TWO-STAGE IMPRESSION TECHNIQUE WITH A SPACER ON DIES AND TOOTHLESS SPACE

To produce a spacer, 2-mm thick acryl was used on the dies, and the toothless space was elevated. A caliper was used to measure the thickness.

MAKING SPACER FOR TWO-STAGE IMPRESSION TECHNIQUE WITH A SPACER ON THE DIES

To make this spacer, both dies were covered with a 2-mm thick acryl.

IMPRESSION STAGE

The main model was subjected to 25°C for about 30 min, when the experiment began. The impression material used was the additional silicon (Betasil, Light, Muller Omicron, Germany). The putty was prepared according to the manufacturer’s instruction: a base materials of putty was mixed by activator. Also, since the latex material may prevent polymerization of additional silicon, mixing was performed without a latex gloves.
The wash (regular set) was made by the following procedure: after placing the cartridge in the injection gun and putting on the mixer head, pressure was applied on the trigger. In this way, the base and catalyst materials were mixed, and a homogeneous mixture was obtained from the injection gun. The setting time was 5 min, which was considered 10 min due to in vitro environment.

ONE-STAGE IMPRESSION TECHNIQUE

Putty and wash were used simultaneously in this technique, where the wash material was prepared concurrently with putty. The putty was then placed inside the tray, while the wash was injected directly around the dies. The experimental model was placed under pressure of 10 psi for 10 min (Figure 2). Once the impression material was set, the tray was separated from the model.

TWO-STAGE IMPRESSION WITH SPACER ON DIES AND TOOTHLESS SPACE

In this technique, the pre-prepared spacer was incorporated and the putty was put inside the specific tray, and the model was impressioned in the presence of the spacer. Then, it was placed under pressure for 10 min. After setting, the spacer was removed and the wash was injected around the dies and in the toothless gap. The die was then placed in and underwent a pressure for 10 min.

TWO-STAGE IMPRESSION WITH SPACER ON DIES

In this technique, the spacer was placed on the dies. Then, the putty was molded by a specific tray and underwent a pressure for 10 min. Once the putty was set, the spacer was removed from the dies and wash was injected around the dies, with the mold embedded in its place. Again, the mold was pressed for 10 min.

INVESTIGATION OF THE EFFECT OF HOLDING TIME ON DIMENSIONAL ACCURACY OF MOLDS

To investigate the effect of holding time on the dimensional accuracy, each of the main groups of molds (each group of 48 samples) was divided into 4 subgroups (12 samples) and stored for 1 h, 24 h, 48 h, and 1 week at 25°C and humidity of 30%. Then, the casts were prepared.

CASTING

The molds were cast with type IV stone (Ariadent, Tahran, Iran). The mixing of stone and water was performed according to the manufacturer’s instruction. First, the stone was mixed manually and then mechanically mixed in the vacuum machine for 15 s. Finally, it was poured into the molds under vibration. The molds were removed from the casts 1 h after casting (Figure 3).

MEASURING THE DIMENSIONS OF CASTS

Measurements of casts included diameter and height of the smaller die and the distance between the two dies. The distance between the two dies was measured by a digital caliper (Mitutoy, Japan), with a measurement accuracy of 0.01 mm. Also, the diameter and height of the smaller die were measured by a digital micrometer (Mitutoy, Japan), with a measurement accuracy of 0.001 mm (Figure 4).

STATISTICAL ANALYSIS

Data were analyzed using IBM SPSS Statistics for Windows Software, version 17 (IBM Corp., Armonk, NY, USA) and descriptive statistic with ANOVA test was used. Statistical significance was set at p < 0.05.

RESULTS

First, the normal distribution of data was evaluated using the Kolmogorov-Smirnov test. The results showed normal distribution of data (p > 0.05). Table 1 reports the results of the mean diameter and height of the smaller die and the distance between the two dies across different impression methods in the gypsum casts.
According to ANOVA, there was a significant difference between various impression methods when examining the three indices (diameter and height of the smaller die as well as the distance between two dies) (p value < 0.05). Based on Scheffe post hoc test, there was a significant difference between one-stage and two-stage methods across all three dimensions. However, there was no significant difference between the two-stage method with spacer on the die, and with spacer on the die and the toothless gap (p value > 0.05).
Mean diameter and height of the smaller die and distance between the two dies at different holding times of the molds are presented in Table 2. According to ANOVA, there was no significant difference between different holding times of dies templates for any dimension (p > 0.05).
According to the comparison of mean numbers obtained from the casts with the dimensions of main model, the accuracy of two-stage impression method was higher than that of one-stage one. This means that the measurements obtained from two-stage impression methods were closer to dimensions of the original model.

DISCUSSION

In fixed prosthodontics treatments, it is essential to provide an accurate mold to obtain appropriate restorations. An impression technique plays a decisive role in the quality of molds obtained. If the impression technique is not as accurate as required, the mold will undergo dimensional changes, and the restoration will not match appropriately [6].
In this study, the accuracy of three different impression techniques was studied (one stage, two-stage with spacer on the dies, and two-stage with spacer on the dies and toothless gap). Four different holding times were investigated for the mold (1 h, 24 h, 48 h, and one week) before casting. The dimensions measured included the dimension and height of the smaller die and the distance between the two dies. The results revealed a significant difference between one- and two-stage methods, where the accuracy of two-stage impression was higher than that of one-stage. However, no significant difference was found between the two-stage methods with various spacers. According to numerous studies, the addition silicon and polyvinyl siloxanes are the most accurate impression materials in fixed restorations regarding holding time of a mold. These materials have a minimum variations when a delay occurs in casting or recasting [9-11]. In the study, statistical analysis showed no significant difference between various holding times. Different impression methods had no mutual effects on each other either. In a similar study by Caputi et al. comparing the dimensional accuracy of one-stage, two-stage with a 2 mm spacer, and two-stage injection impression method, it was concluded that two-stage method with spacer and two-stage injection method were more accurate than one-stage method, which is consistent with our present study [12]. In another study, the dimensional accuracy of three different impression methods was investigated, which used polyvinyl siloxane: one-stage method, two-stage method with 2-mm relief in the putty, and two-stage method with polyethylene spacer. They finally concluded that this two-stage method with 2-mm relief in the putty was the most accurate [8]. The results were somehow similar to findings of the present study.
In another similar study, comparison of the effect of three impression methods on the dimensional varia-tion of dies indicated that impression techniques had an impact on the dimensional accuracy, where two-stage impression technique with a 2-mm spacer was more accurate than two-stage method without spacer and one-stage method [13]. Although the material used in this study was a condensation silicone impression (Speedex), the method of spacing and its volume (wash thickness) were similar to our study. The study of two two-stage method with a foil spacer and a putty cutout method suggested that the two-stage impression with foil spacer (61.4 to 64.4 mm) was more accurate than the cut out two-stage method (55.4 to 61.4 mm) [14]. The reason for the accuracy of two-stage method with a foil spacer was the uniform thickness of wash, which is somewhat similar to that of our study.
Comparison of the dimensional accuracy of one-stage method with the putty-wash two-stage method with 5 types of addition silicone, in which a metal tray was used demonstrates that the dimensional accuracy was more influenced by the type of impression material than by the impression method. Further, only in one of the measured dimensions, the one-stage method had a higher accuracy compared to its two-stage counterpart [15]. The results of this study contradict our findings. The reason for this discrepancy can be the type of impression material, impression technique, and in vitro conditions.
Rathee et al. investigated the dimensional accuracy of addition silicon in impression with various spacers. Two spacers were used for molding, one only covering the die and the other covering the die and the toothless gap. The authors concluded that the impression method, in which the spacer covered the die and the toothless gap simultaneously was more accurate [16]. The results of this study have not been consistent with ours. The different impression material brand (Dentsply) and in vitro conditions can be the reasons for inconsistency. In another study, the effect of three impression methods was investigated on the dimensional accuracy of die. In this study, the in vitro model consisted of three pillars and a type of addition silicon called Affinis used as impression material. Three techniques were used to prepare the molds: one-stage, two-stage without spacer, and two-stage with spacer. The final casts were measured by a profilometer microscope and a micrometer. The authors observed that impression methods did not affect dimensional variations [7]. The results of this study have not been consistent with our findings. The difference between the impression material brand and different in vitro conditions could be the reasons for inconsistency.
Idris et al. conducted a comparative study on the effects of one- and two-stage impression methods on the dimensional accuracy of die. For each method, 15 molds were prepared and cast. They concluded that variations were clinically negligible and that differences between various impression methods were not significant [17]. The results of this study have not been consistent with the present study outcomes. In this study, a special metal tray was used, which could minimize mold variations due to hardness. However, in our study, a specific acrylic tray was used.
A similar study was conducted by Mosharraf et al. on the effect of holding time on dimensional variations of polyvinyl siloxane impression material. In this study, an in vitro model consisting of three pillars was prepared. The molds were divided into four 10-member groups, with each cast at 1, 4, 24, and 48 h post-molding. Statistical analysis did not show any significant difference between these four groups [18]. However, the results have been compatible with our findings.
Thongthammachat et al. studied the dimensional accuracy of casts for different types of tray, impression materials, and holing times. In this study, four different types of materials were used to make the tray, two types of materials for molding, and four different holding times for molds (30 min, 6 h, 24 h, and 30 days). The two materials used for impression included polyether and addition silicon. After measuring the dimensions, they concluded that the addition silicon did not undergo dimensional changes until 30 days after impression (change is negligible) [19]. The result of this study was consistent with our observations. Levartovsky et al. investigated the effect of one-stage and two-stage impressions using the addition silicon as well as different holding times of molds. They prepared 15 casts in each method, where the holding durations of the molds were 0.5, 2, 24, 48, 72, 96, and 120 h. Measurements were done by a microscope. They concluded that the two-stage impression method was more accurate than the single-stage one. In case of the one-stage method, the molds should have been casted for 2 h, but in a two-step method, the casts would not experience dimensional variations even until 30 h [20]. The results of this study have not been consistent with our study in terms of the mutual effects of time and the impression method. However, they have been similar concerning the accuracies. The differences can be due to the difference in the brand of addition silicone (President) and in vitro conditions.
In another study, the effect of holding time was investigated on the viscoelastic properties of elastomeric impression materials. Six types of impression materials from different brands (five types of silicones [compression or addition] and one type of polyether) were used. After being stored for 30 min to 2 weeks, they were tested both statically and dynamically. The results revealed that the holding time had a significant effect on their viscoelastic properties. All of these impression materials exhibited linear viscoelastic behavior and they underwent permanent deformation after 50 h [3]. The results of this study were dissimilar with our results. The tests in this study were performed on molds, while in the present study, they were performed on die casts. Dimensional changes of stone (stone brand, powder to water ratio, in vitro storage conditions) could also affect the results.
Considering the importance of obtaining an accurate cast in the success of prosthodontics, it is recommended that further studies are performed to investigate the effects of other impression techniques, different holding times of mold, and various prosthodontic treatments (fixed prosthodontic, partial prosthodontic, implant). It is also recommended to study other factors affecting the success of fixed prosthodontics, such as waxing up, casting, and porcelain.

CONCLUSIONS

Considering the limitation and conditions of the study, two-stage impression could result in casts with higher dimensional accuracy than their one-stage counterparts. Also, in case of using addition silicones, impression could be postponed by one week without significant dimensional changes in the final cast.

CONFLICT OF INTEREST

The authors declare no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

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