eISSN: 1897-4252
ISSN: 1731-5530
Kardiochirurgia i Torakochirurgia Polska/Polish Journal of Thoracic and Cardiovascular Surgery
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3/2023
vol. 20
 
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abstract:
Original paper

Effects of hypoxia versus ischaemia on vascular functions of isolated rat thoracic aorta: revisiting the in vitro vascular ischaemia/reperfusion model

Halit Güner Orhan
1
,
Ariyan Teimoori
1
,
Elif Demirtaş
1
,
Nargiz Zeynalova
1
,
Oğuzhan Ekin Efe
1
,
Selda Emre Aydingöz
1

  1. Department of Medical Pharmacology, Başkent University Faculty of Medicine, Ankara, Turkey
Kardiochirurgia i Torakochirurgia Polska 2023; 20 (3): 173-178
DOI: https://doi.org/10.5114/kitp.2023.131952
Online publish date: 2023/10/30
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Introduction
The in vitro rat vascular ischaemia and reperfusion model is used to evaluate the molecular and functional effects of potential agents against ischaemia and reperfusion injury of autologous graft veins. However, there is no consensus on whether hypoxia, rather than ischaemia, is sufficient to induce vascular dysfunction.

Aim
To compare the effects of hypoxia and ischaemia, with or without reperfusion, on the vascular functions of isolated thoracic aortic rings of rats.

Material and methods
Thoracic aortas of 12 male Sprague-Dawley rats (350–500 g, 18–24 months old) were isolated and divided into rings that were randomly allocated to control, ischaemia, hypoxia, ischaemia-reperfusion, and hypoxia-reperfusion groups. Aortic rings other than those of the control group were stored at 4°C for 24 h in saline. For ischaemia, saline was gassed with nitrogen. After 24 h, aortic rings in the ischaemia-reperfusion and hypoxia-reperfusion groups were incubated with 200 µM sodium hypochlorite for 30 min. Vascular and endothelial functions were tested in an organ bath set-up.

Results
Vascular response to potassium chloride (80 mM) decreased in all experimental groups compared to the control group (p = 0.007), but phenylephrine-induced contraction (10–5 M) increased only in the ischaemia-reperfusion group (p < 0.0001). Acetylcholine (10–11–10–5 M)-induced endothelium-dependent vasorelaxations were impaired in all groups – particularly in the ischaemia-reperfusion group (p = 0.0011). Sodium nitroprusside (10–12–10–7 M)-induced endothelium-independent vasorelaxations were similar across all groups (p = 0.1258).

Conclusions
Ischaemia followed by reperfusion should be implanted to achieve maximum endothelial and contractile dysfunction in vitro, and to replicate ischaemia and reperfusion injury of autologous graft veins.

keywords:

endothelial function, hypoxia, in vitro, ischaemia, reperfusion
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