Experimental immunology
Sarcoma L-1 in mice as a model for the study of experimental angiogenesis

In this paper animal model for in vivo study of angiogenesis stimulators and inhibitors is described. The L-1 sarcoma, tumor which spontaneously arose in the lung of Balb/c mouse is maintained in vivo and was adapted to the growth in vitro. The L-1 cells from in vitro culture after injection under the Balb/c mice skin form tumors which then could be further transplanted, and used in the experiments in which we can observed vascularization, tumor growth and hemoglobin content in the tumors. The attitude of this model is that we used the same L-1 sarcoma cells in consecutive experiments from the stock L-1 cells obtained from in vitro culture, and saved in LN2. The paper describes some parameters of activity of L-1 sarcoma cells taken from LN2 collection, after various in vivo passages. Angiogenic activity of tumor cells was comparable in all studied passages except that it is a little higher during the third passage. The highest blood supply was present in the tumors during the second and fifth passages. The highest growth L-1 tumors during 14-days was observed in the third passage. For TIA tests cell suspensions from the passage 2nd to the passage 6th may be used interchangeably. However, use of the tumors of similar mass (about 300 mg) is recommended what should assure better standardization of the method.
L-1 sarcoma cells from 4th passage were used for estimation of the effect of two low-molecular weight heparins, enoxaparine and nadroparine, on tumor growth after subcutaneous grafting and on neovascular reaction after intradermal injection of these cells. Nadroparine significantly increased, and enoxaparine significantly decreased neovascular reaction and tumor growth.