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No association between the brain-derived neurotrophic factor 196G>A or 270C>T polymorphisms and Alzheimer’s or Parkinson’s disease
Marika S. Saarela, Terho Lehtimaki, Juha O. Rinne, Heini Huhtala, Riikka Rontu, Antti Hervonen, Matias Röyttä, Jukka-Pekka Ahonen, Kari M. Mattila
Folia Neuropathol 2006; 44 (1): 12-16
- No association.pdf [0.07 MB]
Alzheimer’s disease (AD) and Parkinson’s disease (PD) are the two most common neurodegenerative diseases sharing the feature of accumulation of abnormal proteins in the brain. Typical neuropathological findings in AD include β-amyloid (Aβ) containing neuritic plaques surrounded by activated microglia that release cytokines and neurofibrillary tangles composed of modified tau and reactive astrocytosis. A possibly more significant neuropathological characteristic of AD is the degeneration of the basal forebrain cholinergic neurons crucially involved in memory and cognition. The appearance of α-synuclein containing Lewy bodies and the loss of dopamine neurons in the substantia nigra are, in turn, typical neuropathological findings in PD . The etiology and pathogenesis of these two conditions still remain largely unknown. The tendency to develop AD and PD runs in some families, suggesting that in these cases there is a significant genetic component elevating the risk of disease. In AD, three genes are known to be responsible for familiar forms of the disease, i.e. amyloid precursor protein and presenilins 1 and 2. Inherited forms, however, cover only 5% of all AD cases and the rest, known as sporadic AD cases, have a more complex etiology due to both environmental and genetic factors. The major known risk factor for sporadic AD is the ε4 allele of apolipoprotein E (APOE) . In PD the mutated forms of α-synuclein gene, ubiquitin C-terminal hydrolase and parkin genes account for rare forms of familial PD. The brain-derived neurotrophic factor (BDNF) is a neurotrophin promoting survival, differentiation and maintenance of neurons . A number of studies have shown decreased BDNF protein and mRNA levels in the brains of AD and PD patients [3,5,8,9,11,18,24,26], suggesting that BDNF may contribute to the development of these disorders. Indeed, two single nucleotide polymorphisms of the BDNF gene, namely 196G>A and 270C>T, have been associated with AD [14,27,30] and PD [20,25,29]. In the present study we tested the hypothesis that the functional BDNF genotypes might affect the risk of AD or PD. We therefore identified the genotypes of BDNF 196G>A and 270C>T polymorphisms in Finnish AD, PD and control patients.
Material and methods
The AD group comprised 97 sporadic late-onset (>65 years) type AD patients (43 with probable and 54 with confirmed AD; 68 women, 29 men). The clinical diagnosis of AD was established according to the report of the National Institute of Neurological and Communicative Disorders and Stroke and the Alzheimer’s Disease and Related Disorders Association (NINCDS-ADRDA) Work Group . The definitive diagnosis of AD (56% of patients) was based on the Consortium to Establish a Registry for Alzheimer’s Disease (CERAD) criteria . The PD group consisted of 52 PD patients (27 women, 25 men; mean age at onset 76.2±6.0 years). The pathological diagnosis of PD (100% of patients) was based on the loss of pigmented neurons in the substantia nigra with gliosis, the presence of pigmented phagocytosis and Lewy bodies, confirming the clinical diagnosis of PD. The 101 controls (56 women, 45 men; mean age at examination or at death 79.0±9.3 years; 58% neuropathologically confirmed) were cognitively healthy subjects with no clinical signs of a neurological or psychiatric disease. Ethical approval for the investigation was obtained from the local Hospital Ethical Committees. Genomic DNA was extracted by standard methods from the whole blood and autopsy-derived frozen brain tissue. The genotyping of the BDNF 196G>A polymorphism was done using ABI Prism 7000 Sequence Detection System (Applied Biosystems, Foster City, CA, USA). The system uses 5’ nuclease assay and fluorogenic allele-specific oligonucleotide probes with conjugated minor groove binder (MGB) group. The nucleotide sequences of the primers and probes used in PCR were deduced from published sequences deposited in the GenBank database and were chosen and synthesized in conjugation with Applied Biosystems (Foster City, CA, USA). PCR was performed in 384-well plates using the standard protocol for TaqMan MGB probes in a total volume of 5 µl. Reagents included genomic DNA, 1 ´ Universal PCR Master Mix, 900 nM of each primer and 200 nM of each probe. Negative controls were run in parallel with unknown DNA samples. After cycling, end-point fluorescence was measured and the allelic discrimination analysis module carried out genotype identification. The genotypes of the BDNF 270C>T and APOE polymorphisms were determined as previously described [7,14,30] using polymerase chain reaction (PCR), digestion with restriction endonucleases and standard agarose gel electrophoresis. The test for Hardy-Weinberg equilibrium was calculated using Arlequin, version 2.000 (Genetics and Biometry Laboratory, Department of Anthropology, University of Geneva, Switzerland, 2000). The genotype and allele frequencies were compared between cases and controls using the χ2 test. In view of the simultaneous comparisons, the correction factor n (m-1) (n loci with m alleles each) was applied to correct the significance level (p<0.008). The effects of BDNF 196G>A and 270C>T polymorphisms on the risk of AD were investigated by the logistic regression analysis (SPSS 12.0.1 for Windows, SPSS Inc. 2003). Age and gender were used for adjustment. The combined effects of APOE ε4 (ε4 carriage/non-carriage) and BDNF 196G>A and 270C>T polymorphisms on the risk of AD were investigated by the logistic regression analysis (SPSS 12.0.1 for Windows, SPSS Inc. 2003).
All patient and control groups for both the BDNF polymorphisms investigated were found to be in the Hardy-Weinberg equilibrium. The distributions of genotypes and allelic frequencies of the BDNF polymorphisms are shown in Table I. The genotype or allele frequencies of 196G>A or 270C>T polymorphisms did not differ significantly between AD, PD and control groups after correction for multiple testing although the difference between the AD and the control groups came close to statistical significance in both of the polymorphisms analyzed (p=0.028, p=0.053). The risk of AD conferred by APOE ε4 was of the same order of magnitude as reported previously (OR: 10.8, CI: 5.6-20.9). No interaction was observed between either of the polymorphisms analyzed and APOE ε4 in AD patients. Discussion In this investigation we found no association between BDNF 196G>A (Val66Met) or BDNF 270C>T polymorphism and AD or PD. Our results confirm the results of another Finnish study , a Spanish study  and several North American studies [1,4] where no association with BDNF 196G>A (Val66Met) polymorphism was found among AD patients. In the American studies, too, no association with BDNF 270C>T polymorphism was found [1,4]. Moreover, the allele frequencies observed in the present study were similar to those in the studies mentioned above [1,2,4,31]. We were not able to replicate previous results where an association between AD and BDNF was found [14,16,23,27,30]. For PD, too, and BDNF 196G>A our result is consistent with another Scandinavian study  and one Japanese study . Our result contradicts those by Japanese researchers [15,20] and the North American researchers . The allele frequencies in these studies differ significantly, the G-allele frequency ranging from 0.47-0.61 [10,15,20] in Asian PD patients to a frequency of 0.82-0.90  in Scandinavian PD patients. As for control patients the G-allele frequencies also differ, ranging from 0.50-0.60 in Asians and 0.82-0.90 in Scandinavians [6,10,12,15,20,22]. This might suggest that there are ethnic differences between Asians and Caucasians in BDNF 196G>A allele distribution. Unlike the North Americans  we found no association between BDNF 270C>T polymorphism and PD, but the number of PD patients in our study was relatively small. The C-allele frequency of PD patients in our study was 0.89 and in the North American patients 0.79 among familial PD patients and 0.85 among sporadic PD patients . In the case of association studies there is always a possibility that the association found is not real but rather due to a linkage disequilibrium with a functional polymorphism in or near the gene studied. This may be the case with BDNF, given the different results reported by numerous researchers. False negative results may also be obtained because of the small sample size. Although our sample size was quite small we could confirm the results of another Finnish study where no association between AD and BDNF, was found . In conclusion, it seems that the BDNF gene is not a significant risk gene among Finnish AD patients.
The authors would like to thank Juhani Salo, neuropsychologist, for his contribution to the study. This work was supported by the Medical Research Fund of Tampere University Hospital.
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