Folia Neuropathologica
 
1/2012
 
Share:
Share:
more
 
 

Review paper
Association of the PRNP regulatory region polymorphisms with the occurrence of sporadic Creutzfeldt-Jakob disease

Jolanta Bratosiewicz-Wąsik, Joanna Smoleń-Dzirba, Cezary Watała, Annemieke J. Rozemuller, Casper Jansen, Wim Spliet, Gerard H. Jansen, Tomasz J. Wąsik, Paweł P. Liberski

Folia Neuropathol 2012; 50 (1): 68-73
documents in PDF format:
 

Introduction



Creutzfeldt-Jakob disease (CJD) is a rare fatal neurodegenerative disease associated with accumulation of the host-encoded abnormal isoform of the prion protein (PrPSc) in the brain and other tissues of affected individuals. The disease may be acquired (kuru and iatrogenic Creutzfeldt-Jakob disease), inherited, or of idiopathic (sporadic) origin [2,8]. The aetiology of the idiopathic form, sporadic Creutzfeldt-Jakob disease (sCJD), remains obscure. However, the prion protein (PRNP) gene polymorphism, encoding methionine or valine at codon 129 is a strong susceptibility factor for sCJD. Patients with sporadic CJD are predominantly methionine or valine homozygous at codon 129 [11] whereas valine homozygosity acts as a susceptibility factor for iatrogenic CJD [7]. However, epidemiological data on sCJD incidence and codon 129 allelic frequencies [1] strongly indicate that this polymorphism cannot be the only factor influencing aetiology of sporadic CJD; therefore, factor or factors other than the coding region of PRNP polymorphisms must be involved in this process. Furthermore, it is believed that, in addition to such factors as the environmental exposition to prions, stochastic conversion of normal prion protein or a so­ma­tic PRNP mutation (albeit never found), which change PRNP expression leading to increase in PrP concentration is influencing susceptibility to sCJD. This suggestion comes from the studies of the transgenic mouse model which demonstrated that the level of PrP gene expression had a significant impact on the initiation and progression of the prion diseases. Transgenic mice containing multiple copies of hamster PrP gene had significantly reduced incubation time after hamster scrapie challenge, otherwise not pathoge­nic to mice, compared with a analogous challenge to wild-type mice [14], while hemizygous mice with only one copy of the PrP gene and with 50% normal PrP expression level had extended incubation period when compared with wild-type mice and Prnp0/0 mice that did not express prion protein were completely resistant to infection with prions [6]. Different PrP expression levels could be due to the polymorphisms of the regulatory region of the PRNP gene. To date, several single nucleotide polymorphisms of the promoter region of the PRNP gene have been reported, a C to G transversion at position –101, a G to C transversion at position +310 and T to C transition at position +385. However, the links of these polymorphisms with the CJD development are inconsistent [10,12,15]. We have previously shown that the presence of –101G allele of PRNP may be considered as the risk factor for sCJD among codon 129 heterozygotes [4]. The purpose of this study was to reveal the possible links between SNPs located outside of the coding region of the PRNP gene and the overall susceptibility to sCJD in the Dutch population. To achieve this goal we investigated the genotype, allele and haplotype frequencies of the following PRNP polymorphisms: +258G/A, +310 G/C, and +385T/C in a sample of 45 patients with sporadic CJD and in 135 control individuals.

Material and methods



Subjects

The study included 45 neuropathologically confirmed sCJD cases of the Dutch origin. The control comprised 135 unrelated individuals with no history of neurodegenerative disease. All patients and controls were Caucasians. Informed consent for research purposes was obtained from all control subjects involved in the study and the project was approved by a local research ethics board. The patients in this study were diagnosed with the sporadic form of CJD and were classified as definite or probable according to criteria established earlier [5]. We sequenced the entire open reading frame of PRNP of all CJD cases to exclude from study the patients with the mutations in the prion protein gene.

Genotyping

Genomic DNA from peripheral blood leukocytes of controls and from post mortem brain tissue samples of CJD cases was isolated by the routine proteinase K-phenol-chloroform extraction. Polymorphism of codon 129 of the PRNP gene and polymorphism of –101 position was examined according to procedures described earlier [4]. The region downstream of exon I spanning nucleotides from 12742 to 13318 (GeneBank U29185) was amplified using the forward primer 5’-TTCTCCTCTCCTCACGACCG and the reverse primer 5’-TCTCTCCATCCCCCCAAGC. The PCR products were purified and then directly sequenced on an ABI 377 automatic sequencer using ABI Prism BigDye Terminator v 3.1 cycle sequencing kit (Applied Biosystems, USA). The results were analyzed using DNA Sequencing Analysis Software version 3.4.1.

Statistical analysis

Hardy-Weinberg equilibrium of all SNPs was determined in controls using a chi-squared test. The association between the rare alleles of PRNP regulatory region in SNPs carriers and the risk of sCJD onset was further analysed. Differences in allele and genotype distribution between cases and controls were tested using Fisher’s exact test within STATISTICA StatSoft 8.2. Genetic association was expressed as odds ratio (OR) for heterozygotes and homozygotes of the rare allele versus homozygotes of the common allele.

To assess the association between sCJD and SNPs haplotypes, we used the Haplo.Stat program, which implements the test proposed by Schaid et al. [13]. Haplotypes with an estimated frequency lower than 0.01 were excluded from further analysis. The standardized measure of linkage disequilibrium (LD) was computed at pairs of SNP loci. Pairwise LD estimations were performed using Haploview software [3].

Haplotypes in two blocks were analyzed separately when D’ > 0.9. Since frequency of minor allele at marker +385 was very low, haplotype analysis within block 2 was limited to polymorphism +285 and +310.

Results



Genotype and allele distribution

To examine the correlation between PRNP non-cod­ing region polymorphisms and susceptibility to sporadic CJD (spanning nucleotides from 12742 to 13318 [Gene­Bank U29185]), we analyzed the genotype and allele frequencies of these polymorphisms and codon 129 poly­morphism in 45 sCJD cases and 135 healthy controls. All genotype frequencies were in Hardy-Weinberg equilibrium (HWE) with the exception of the PRNP codon 129 polymorphism in controls (P = 0.001). This variation from HWE confuses the haplotype analyses and interpretation of these SNP results.

Distribution of codon 129 polymorphism of the PRNP gene was as follows: 15 (33%) CJD cases and 86 (64%) control individuals were M/V heterozygous, 23 (51%) CJD cases and 30 (22%) control individuals were M/M homozygous, and 7 (16%) CJD cases and 19 (14%) control individuals were V/V homozygous. These results are consistent with the earlier reports that codon 129 M/M homozygosity acts as a susceptibility factor whereas heterozygosity at codon 129 partially protects against development of sCJD (OR 4.40; 95% CI 2.03-9.51; P = 0.0002).

We found four single nucleotide polymorphisms within regulatory region of the PRNP gene: C to G transversion at position –101 occurring at an allele frequency of 0.11 in control individuals and 0.16 in CJD cases, G to A substitution at position +258 at an allele frequen­cy of 0.03 in control individuals and 0.01 in CJD cases, G to C transversion at position +310 at an allele frequency of 0.15 in control individuals and 0.04 in CJD cases, and T to C substitution at position +385 at an allele frequency of 0.01 in control individuals (+385 C allele was not found in CJD cases).

Association of SNPs with sCJD

As shown in Table I, there were no statistical association between –101C/G (OR = 1.54, 95% CI: 0.79-3.01; P = 0.21 for G vs. C allele and OR = 1.96, 95% CI: 0.93-4.16; P = 0.1 for C/G vs. C/C genotype) as well as +258 G/A (OR = 0.37, 95% CI: 0.45-2.98; P = 0.46 for A vs. G allele and OR = 0.36, 95% CI: 0.44-2.97; P = 0.45 for G/A vs. G/G genotype) polymorphisms and CJD susceptibility. Significantly decreased ORs were found in +310 C allele (OR = 0.27, 95% CI: 0.09-0.77; P = 0.009) and +310 GC genotype (OR = 0.33, 95% CI: 0.11-0.98; P = 0.048) carriers.

Haplotype frequency and linkage disequilibrium

Two haplotype blocks were constructed using Hap­­loview, an LD-based partitioning algorithm, as shown in Fig. 1. Haplotype in two blocks was analyzed separately when D’ value was over 0.9. Since frequency of minor allele of +385 position was very low, haplotype analysis within block 2 was limited to polymorphisms +258 and +310. Haplotypes with an estimated frequency lower than 0.01 were excluded. As shown in Table II, there was a significant negative association between haplotype C-V in block 1 and sCJD (Score = –2.47, P = 0.014). In block 2, haplotype G-C was associated with a reduced risk of sCJD (Score = –2.46, P = 0.014) whereas haplotype G-G was associated with an in­creased risk of sCJD (Score = 2.77, P = 0.006).

Discussion



Consistently with the earlier published data we found 129Met/Met homozygosity associated with an increased risk of sCJD (OR = 4.40, 95% CI: 2.03-9.51; P = 0.0002). However, the interpretation of these results is hindered by deviation of controls from HWE. Genotype frequencies may deviate from the expected values for several reasons such as genotyping error, small population size due to genetic drift and founder effect, and nonrandom mating. 129Met/Val PRNP polymorphism is a widely do­cumented susceptibility sCJD factor but it does not explain all sCJD cases. Recently a lot of effort was put into identification of other genetic risk factors, especially located within the PRNP regulatory regions. Previous studies of non-coding regions of the PRNP gene showed presence of the regulatory sequence upstream of the transcription start site between –148 and –114 [9]. In addition, the intronic region of PRNP was proved to contain regulatory elements between +292 and +625 positions [10]. An association of sCJD with the polymorphisms in the regulatory regions of PRNP was investigated by several authors.

Analysis of studies of the association of –101 C/G polymorphism with sCJD lead to ambiguous conclusions. Previously we have found that -101G allele acts as a risk factor in codon 129 heterozygous subgroup (P = 0.047) [4]. In the present study we demonstrated a slight overrepresentation of –101G allele and –101G/C heterozygosity in the sCJD group, however these differences were not statistically significant. The influence of polymorphisms located within the PRNP non-coding regions was studied by McCormack et al. [10], who observed a non-significant overrepresentation of –101G allele in 129Met homozygous sCJD patients compared with controls. In this study, –101G allele seemed to be a susceptibility factor in 129Met homozygotes when the number of its carriers was compared with the number of individuals homozygous for the common allele at polymorphisms tested. On the contrary, no association between –101 PRNP polymorphism and sCJD was shown by Vollmert et al. in the study involving 534 sCJD cases and 740 controls [15].

The presented work showed deficiency of +258A allele among the sCJD group, however this difference was not statistically significant. We also found no association of +385 polymorphism with sCJD, though the rare allele occurs with very low frequency (+385C allele 0.007), which is in accordance with McCormack’s re­port showing non-significant excess of +385C allele in sCJD129Met/Met homozygotes compared to an ana­-logous control subgroup.

While evaluating the association of +310G/C SNP with sCJD in the Dutch population, we have found a significant relation of +310 G/C SNP with the sporadic form of CJD. The presence of +310 G/C genotype results in an almost three-fold decreased risk of sCJD (OR = 0.33, 95% CI: 0.11-0.98; P = 0.048). This effect is even more noticeable if +310C allele frequency was compared (OR = 0.27, 95% CI: 0.09-0.77; P = 0.009).

McCormack et al. found a non-significant overr­e­presentation of +310C allele carriers among 129Met/Met homozygous sCJD patients compared to 129Met/Met control subgroup. However, their study comprised limited numbers of subjects (25 sCJD cases and 100 controls). Interestingly, Sanchez-Juan et al. recently found that carrying +310 C allele is associated with 2.4 increase of developing sCJD when adjusted with PRNP 129Met/Val genotype [12].

Here, we demonstrated that five SNPs are formed into two haploblocks. We found two haplotypes protecting from sCJD (C-V in block 1 and G-C in block 2) and one susceptible haplotype for sCJD (G-G in block 2).

Because the genetic variations of the PRNP gene leading to alterations of the protein structure are involved in minority of CJD cases, polymorphisms in the regulatory region of the prion protein gene are considered as the CJD susceptibility factors. The study of the regulatory region of the PRNP gene may provide a key to solving the problem of individual susceptibility to sporadic and acquired forms of prion diseases. Studies of a correlation between PRNP polymorphisms and CJD in different populations revealed contradictory results. There may be various explanations for these differences across studies. One possibility may be a re­latively small sample size of CJD patients and control subjects. The other explanation is that the distribution of the PRNP genotype between studied ethnic groups is different. Further meta-analysis is needed to solve this enigma. Moreover, the research on the physiological role of PRNP promoter polymorphisms including their influence on PrP expression levels should be performed in the future.

Acknowledgements



The study was supported by Ministry of Scienti­fic Research and Information Technology grant No. PBZ-KBN-124/P05/2004. We thank Piotr Kruszyński for his technical assistance in this project.

References



 1. Alperovitch A, Zerr I, Pocchiari M, Mitrova E, de Pedro Cuesta J, Hegyi I, Collins S, Kretzschmar H, van Duijn C, Will RG. Codon 129 prion protein genotype and sporadic Creutzfeldt-Jakob disease. Lancet 1999; 353: 1673-1674.

 2. Armstrong RA. Dispersion of prion protein deposits around blood vessels in variant Creutzfeldt-Jakob disease. Folia Neuropathol 2010; 48: 150-158.

 3. Barrett JC, Fry B, Maller J, Daly MJ. Haploview: analysis and visualization of LD and haplotype maps. Bioinformatics 2005; 21:

263-265.

 4. Bratosiewicz-Wasik J, Liberski P P, Golanska E, Jansen GH, Wasik TJ. Regulatory sequences of the PRNP gene influence susceptibility to sporadic Creutzfeldt-Jakob disease. Neurosci Lett

2007; 411: 163-167.

 5. Budka H, Aguzzi A, Brown P, Brucher JM, Bugiani O, Gullotta F, Haltia M, Hauw JJ, Ironside JW, Jellinger K, Kretzschmar HA, Lantos PL, Masullo C, Schlote W, Tateishi J, Weller RO. Neuro­pathological diagnostic criteria for Creutzfeldt-Jakob disease (CJD) and other human spongiform encephalopathies (prion diseas­es). Brain Pathol 1995; 5: 459-466.

 6. Büeler H, Aguzzi A, Sailer A, Greiner RA, Autenried P, Aguet M, Weissmann C. Mice devoid of PrP are resistant to scrapie. Cell 1993; 73: 1339-1347.

 7. Collinge J, Palmer MS, Dryden AJ. Genetic predisposition to iatrogenic Creutzfeldt-Jakob disease. Lancet 1991; 337: 1441-1442.

 8. Liberski PP. Kuru and D. Carleton Gajdusek: a close encounter.

Folia Neuropathol 2009; 47: 114-137.

 9. Mahal SP, Asante EA, Antoniou M, Collinge J. Isolation and functional characterisation of the promoter region of the human

prion protein gene. Gene 2001; 268: 105-114.

10. McCormack JE, Baybutt HN, Everington D, Will RG, Ironside JW, Manson JC. PRNP contains both intronic and upstream regulatory regions that may influence susceptibility to Creutzfeldt-Jakob Disease. Gene 2002; 288: 139-146.

11. Palmer MS, Dryden AJ, Hughes JT, Collinge J. Homozygous prion protein genotype predisposes to sporadic Creutzfeldt-Jakob disease. Nature 1991; 352: 340-342.

12. Sanchez-Juan P, Bishop MT, Croes EA, Knight RS, Will RG, van

Duijn CM, Manson JC. A polymorphism in the regulatory region of PRNP is associated with increased risk of sporadic Creutzfeldt-Jakob disease. BMC Med Genet 2011; 12: 73.

13. Schaid DJ, Rowland CM, Tines DE, Jacobson RM, Poland GA. Score tests for association between traits and haplotypes when linkage phase is ambiguous. Am J Hum Genet 2002; 70: 425-434.

14. Scott M, Foster D, Mirenda C, Serban D, Coufal F, Walchli M, Torchia M, Groth D, Carlson G, DeArmond SJ. Transgenic mice expressing hamster prion protein produce species-specific scrapie infectivity and amyloid plaques. Cell 1989; 59: 847-857.

15. Vollmert C, Windl O, Xiang W, Rosenberger A, Zerr I, Wichmann pHE, Bickeböller H, Illig T; KORA group, Kretzschmar HA. Significant association of a M129V independent polymorphism in the 5’ UTR of the PRNP gene with sporadic Creutzfeldt-Jakob disease in a large German case-control study. J Med Genet 2006; 43: e53.
Ten materiał jest chroniony prawami autorskimi. Wykorzystywanie do dalszego rozpowszechniania bez zgody właściciela praw autorskich jest zabronione. Zobacz regulamin korzystania z serwisu www.termedia.pl.
Featured products
Conferences:
KONTROWERSJE W NEUROLOGII 2014
06.06.2014 - 07.06.2014
pozostało 43 dni
Books:
Zawroty głowy (krótkie kompendium)
Bożydar Latkowski, Antoni Prusiński

format A5
liczba stron 140
oprawa miękka
 

NOWOŚĆ!
Cała prawda o e-papierosach

Jean-François Etter, Gérard Mathern

Format: 125x197 mm
Liczba stron: 208
Oprawa: miękka
 
Leksykon schizofrenii
prof. dr hab. med. Marek Jarema





Format B5
Liczba stron 168
Oprawa twarda
 
Leksykon genetyki w psychiatrii
prof. dr hab. n. med. Joanna Hauser, dr n. med. Monika Dmitrzak-Węglarz



Format B5
Liczba stron 168
Oprawa twarda
 
Internet:
Studenci Medycyny i Farmacji
Portal adresowanych do studentów uczelni medycznych w Polsce i za granicą.
Privacy policy Advertising policy Contact us
Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Created by Bentus
PayU - płatności internetowe