eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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3/2022
vol. 75
 
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abstract:
Original paper

Dentinal tubules obliteration using a toothpaste with nano-hydroxyapatite obtained from chicken eggshell

Karina Huamán-Mujica
1
,
José A. Castañeda-Vía
2
,
Vanessa S. Bermúdez García
1
,
John A. Dominguez
1
,
Carlos V. Landauro
3, 4
,
Justiniano Quispe-Marcatoma
3, 4
,
Lidia Y. Tay Chu Jon
1

1.
Facultad de Estomatología, Universidad Peruana Cayetano Heredia, Peru
2.
Escuela de Posgrado “Victor Alzamora Castro”, Universidad Peruana Cayetano Heredia, Peru
3.
Facultad de Ciencias Físicas, Universidad Nacional Mayor de San Marcos, Peru
4.
Línea “Agua, Suelo y Sociedad”, Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales (CITBM), Peru
J Stoma 2022; 75, 3: 147-154
Online publish date: 2022/08/30
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Introduction
Dentin hypersensitivity is a common pathology, produced by exposed dental tubules.

Objectives
The aim of this study was the evaluation of the effectiveness of experimental toothpaste based on nano-hydroxyapatite (nHAP) obtained from chicken eggshell in the obliteration of dentinal tubules (DTs).

Material and methods
Toothpastes with different percentages of nHAP were formulated, including 3%, 7%, and 15% of nHAP, a commercial paste, and a toothpaste without nHAP. Assays were made using healthy premolar samples (n = 50). The pastes were applied and brushed for 7 days after a first erosive cycle, then a second erosive cycle was made at 15 days. Samples were analyzed using scanning electron microscopy and confocal Raman micro­scopy at three times: after the first erosive cycle (T0), after applications of the pastes (T7), and after the second erosive cycle (T15).

Results
The quantitative analysis of the micrographs showed a significant difference between the experimental toothpastes with respect to the controls. Moreover, there was no statistically significant difference between the expe­rimental toothpastes at time T7. By mineral concentration analysis, the 3%-nHAP toothpaste presented a significant difference against conventional toothpaste, when evaluating mineral concentration at 7 days.

Conclusions
The experimental paste is effective in obliterating DTs at concentrations of 7 and 15%, in addition to being stable over time.

keywords:

eggshell, durapatite, hypersensitivity, microscopy, toothpastes

 
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