Abstract
Dentinal tubules obliteration using a toothpaste with nano-hydroxyapatite obtained from chicken eggshell
- Facultad de Estomatología, Universidad Peruana Cayetano Heredia, Peru
- Escuela de Posgrado “Victor Alzamora Castro”, Universidad Peruana Cayetano Heredia, Peru
- Facultad de Ciencias Físicas, Universidad Nacional Mayor de San Marcos, Peru
- Línea “Agua, Suelo y Sociedad”, Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales (CITBM), Peru
Introduction
Dentin hypersensitivity is a common pathology, produced by exposed dental tubules.
Objectives
The aim of this study was the evaluation of the effectiveness of experimental toothpaste based on nano-hydroxyapatite (nHAP) obtained from chicken eggshell in the obliteration of dentinal tubules (DTs).
Material and methods
Toothpastes with different percentages of nHAP were formulated, including 3%, 7%, and 15% of nHAP, a commercial paste, and a toothpaste without nHAP. Assays were made using healthy premolar samples (n = 50). The pastes were applied and brushed for 7 days after a first erosive cycle, then a second erosive cycle was made at 15 days. Samples were analyzed using scanning electron microscopy and confocal Raman microscopy at three times: after the first erosive cycle (T0), after applications of the pastes (T7), and after the second erosive cycle (T15).
Results
The quantitative analysis of the micrographs showed a significant difference between the experimental toothpastes with respect to the controls. Moreover, there was no statistically significant difference between the experimental toothpastes at time T7. By mineral concentration analysis, the 3%-nHAP toothpaste presented a significant difference against conventional toothpaste, when evaluating mineral concentration at 7 days.
Conclusions
The experimental paste is effective in obliterating DTs at concentrations of 7 and 15%, in addition to being stable over time.
>Keywords
eggshell, durapatite, hypersensitivity, microscopy, toothpastes
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