IgE, but not allergens, induces the release of neutrophil extracellular traps

Olga Ciepiela; Weronika Kuźmicka; Katarzyna Lewandowska; Anna Zawadzka-Krajewska; Aneta Manda-Handzlik; Małgorzata Wachowska

doi:10.5114/pja.2023.133769

eISSN: 2391-6052
ISSN: 2353-3854

Alergologia Polska - Polish Journal of Allergology

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4/2023
vol. 10

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abstract:

Original paper

IgE, but not allergens, induces the release of neutrophil extracellular traps

Olga Ciepiela

¹

,

Weronika Kuźmicka

²

,

Katarzyna Lewandowska

³

,

Anna Zawadzka-Krajewska

⁴

,

Aneta Manda-Handzlik

²

,

Małgorzata Wachowska

²

Department of Laboratory Medicine, Medical University of Warsaw, Warsaw, Poland
Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland
Student’s Scientific Group at Department of Laboratory Diagnostics and Clinical Immunology of Developmental Age, Medical University of Warsaw, Warsaw, Poland
Department of Pediatric Allergology and Pneumonology, Medical University of Warsaw, Warsaw, Poland

Alergologia Polska – Polish Journal of Allergology 2023; 10, 4: 249–259

DOI: https://doi.org/10.5114/pja.2023.133769

Online publish date: 2023/12/15

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Introduction:
Neutrophils play an important role in innate immunity. To eliminate pathogens they use several mechanisms, such as phagocytosis, degranulation, production of reactive oxygen species (ROS), and release of neutrophil extracellular traps (NETs). It was recently shown that neutrophils can degranulate under the influence of immunoglobulin E (IgE) as they express receptors for IgE. The impact of IgE on the release of NETs has not been investigated thus far.

Aim:
To investigate if neutrophils can release NETs following IgE, anti-IgE or allergen stimulation.

Material and methods:
Neutrophils were isolated by gradient centrifugation from peripheral blood obtained from sensitized patients and healthy blood donors. Basophil activation was measured using flow cytometry. The presence of NETs following IgE, anti-IgE and allergen stimulation was evaluated quantitatively by fluorometry and qualitatively by fluorescent microscopy.

Results:
IgE stimulated NETs release in a concentration-dependent manner, causing the greatest release at 2.86 µg/ml. NETs release under IgE stimulation was significantly stronger in allergic subjects comparing with non-allergic ones, and seemed to be independent of FcεRI expression. IgE-dependent NETs release was significantly decreased following treatment with N-acetylcysteine and 4-aminobenzoic acid. Anti-IgE and allergens did not affect NETs release.

Conclusions:
Neutrophils can release NETs in the presence of a high concentration of IgE, which appears to be partially dependent on the allergic status of the patient and the production of ROS. This finding may contribute to better understanding of inflammatory processes accompanied by atopic diseases.

keywords:

IgE, but not allergens, induces the release of neutrophil extracellular traps

Olga Ciepiela 1 , Weronika Kuźmicka 2 , Katarzyna Lewandowska 3 , Anna Zawadzka-Krajewska 4 , Aneta Manda-Handzlik 2 , Małgorzata Wachowska 2

allergy, neutrophils, immunoglobulin E, anti-IgE, neutrophil extracellular traps

Olga Ciepiela

¹

,

Weronika Kuźmicka

²

,

Katarzyna Lewandowska

³

,

Anna Zawadzka-Krajewska

⁴

,

Aneta Manda-Handzlik

²

,

Małgorzata Wachowska

²