Introduction

There is a strong need for the personalised precise genotype-associated diagnostic tools to detect the probability (P) of the poly-organ clinical profiles (CP) of atopic march (AM) in children.

Aim

To develop the integral model (IM) of the personalised precise genotype-associated diagnostics (PPGAD) of the poly-organ CPAM development probability in children.

Material and methods

We recruited 21 patients into the study group – children with poly-organ CP comprising atopic dermatitis (AD), bronchial asthma (BA), allergic rhinitis or rhino-conjunctivitis (AR/ARC): AD + AR, AD + ARC, AD + AR + ARC, AD + BA. We recruited 47 patients into the control group – children without any atopic disorders, suffering from digestive tract disorders (DTD). All the patients underwent the genotyping for the single nucleotide variants (SNV) of the filaggrin gene (rs7927894 FLG) and orosomucoid-1 -like protein 3 (rs7216389 ORMDL3) along with detection of the serum concentration levels of total immune globulin E (IgE) and cutaneous T-cell attracting chemokine (CTACK/CCL27).

Results

The integral model (IM) of the personalised precise genotype-associated diagnostics (PPGAD): cut-off value for the increased probability (P) of the poly-organ CP of AM development > 0.403, area under the curve = 0.961 (p < 0.001); specificity = 95.7% (95% CI: 85.5–99.5), sensitivity = 85.7% (95% CI: 63.7–97.0). We developed the designed PPGAD IM on 4 clinical cases. Of the study group patients: with mono-organ CP of AD p = 0.118, with the CP AD + AR + ARC p = 0.986. In the control group patients with DTD: p = 0.027 and p = 0.403.

Conclusions

PPGAD integral model of development probability of the poly-organ CP of AM comprises genotyping the child for the SNV C/Т rs7927894 FLG, Т/Т rs7216389 ORMDL3, detecting serum levels of CTACK/CCL27 and total IgE. It needs further validation in longitudinal studies on larger patients’ cohorts.