Prognostic significance of occurrence of alternative splice mRNA VEGF forms – VEGF₁₂₁, VEGF₁₄₅, VEGF₁₆₅, VEGF₁₈₃, VEGF₁₈₉, VEGF₂₀₆in squamous intraepithelial lesions and cervical cancer

Investigations show, that angiogenesis has largest meaning in process of progression of cervical cancer. Largest proangiogenic working shows VEGF. Well-known is six isoforms, which come into being mRNA: VEGF₁₂₁, VEGF₁₄₅, VEGF₁₆₅, VEGF₁₈₃, VEGF₁₈₉ as well as VEGF₂₀₆on road of alternative maturation. 119 segments (43 segments of proper epithelium, 38 LSIL, 17 HSIL, cervical cancer made up 21 segments in degree of clinical advancement IB – IIB) were pathomorfological estimated and molecular analysis was surrendered. Degree of expression VEGF was estimated (marking quantity of copy mRNA/1 μg RNA in studied tissue). Risk of cancer progression in aspect of occurrence of alternative splice mRNA VEGF forms were estimated. VEGF transcriptive activity in HSIL group was ignificantly higher in comparison with group LSIL and proper tissues of epithelium groups, VEGF transcriptive activity in LSIL group was significantly higher in comparison with proper tissues of epithelium group. Height of VEGF transcriptive activity in studied tissues segments of cervical cancer were not observed. In dependence from affirming isoforms VEGF risk of progression LSIL carries out suitably: for VEGF₁₂₁ – 2.28, for VEGF₁₄₅ – 1.32, for VEGF₁₆₅ – 1.53, for VEGF₁₈₃ – 1.73, for VEGF₁₈₉ – 2.02, for VEGF₂₀₆ – 1.45. In dependence from affirming isoforms VEGF risk of progression HSIL carries out suitably: for VEGF₁₂₁ – 8.94, for VEGF₁₈₉ – 12.95, for VEGF₂₀₆ – 9.81. In dependence from affirming izoformy VEGF risk of progression of cervical cancer carries out suitably: for VEGF₁₂₁ – 9.35, for VEGF₁₆₅ – 5.49, for VEGF₁₈₃ – 4.46, for VEGF₁₈₆ – 3.58, for VEGF₂₀₆ – 21.29.