Experimental immunology Effect of ethanol extract of propolis (EEP) on interleukin 8 release by human gastric adenocarcinoma cells (AGS) infected with 
Helicobacter pylori

Mariusz Skiba; Ewelina Szliszka; Mariola Kunicka; Wojciech Król

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2011
vol. 36

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abstract:

Experimental immunology
Effect of ethanol extract of propolis (EEP) on interleukin 8 release by human gastric adenocarcinoma cells (AGS) infected with Helicobacter pylori

Mariusz Skiba

,

Ewelina Szliszka

,

Mariola Kunicka

,

Wojciech Król

(Centr Eur J Immunol 2011; 36 (2): 65-69)

Online publish date: 2011/06/20

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Mucosal chemokines are considered to be involved in the pathogenesis of Helicobacter pylori associated gastritis as well as gastric adenocarcinoma as a consequence of chronic inflammation process. Interleukin 8 (IL-8) produced by infected epithelial cells plays an important role in active inflammation by the induction of neutrophil migration and activation. The aim of a study was to investigate the effect of ethanol extract of propolis from Poland (EEPP) on IL-8 release by H. pylori infected human gastric adenocarcinoma cells (AGS).

Materials and methods: The AGS line cells was incubated with bacteria (bacteria to cell ratio was 100 : 1) in the absence (control) or the presence of tested solutions of EEPP (7.5-60 g/ml) for 16 hours. The concentrations of IL-8 were determined in culture supernatants by ELISA method.

Results: Incubation of AGS cells in presence of EEPP significantly suppressed IL-8 release in comparison with the control (p < 0.01). Obtained data has shown that IL-8 inhibition of EEPP was dose dependent. Additionally, 90 g/ml of EEPP significantly inhibited growth of H. pylori (p < 0.05). These results indicate that EEPP preparations might partially inhibit H. pylori-induced gastric inflammation by decreasing IL-8 production.

keywords:

Experimental immunologyEffect of ethanol extract of propolis (EEP) on interleukin 8 release by human gastric adenocarcinoma cells (AGS) infected with Helicobacter pylori

Mariusz Skiba , Ewelina Szliszka , Mariola Kunicka , Wojciech Król

propolis, Helicobacter pylori, interleukin 8, AGS cell line

Experimental immunology
Effect of ethanol extract of propolis (EEP) on interleukin 8 release by human gastric adenocarcinoma cells (AGS) infected with Helicobacter pylori

Mariusz Skiba

,

Ewelina Szliszka

,

Mariola Kunicka

,

Wojciech Król