eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Search
1/2011
vol. 36
 
Share:
Send email
Share:
abstract:

Experimental immunology
Iron chloride cytotoxicity and cytokines (IL-1α and IL-6) production in BALB/c mice embryo fibroblasts cell line

Sylwia Terpiłowska
,
Andrzej Krzysztof Siwicki

(Centr Eur J Immunol 2011; 36 (1): 1-4)
Online publish date: 2011/03/29
View full text Get citation
 
The aim of this study was to examine the effect of the iron chloride on mouse fibroblasts. The experiments were performed on the BALB/c 3T3 (mouse BALB/c embryo fibroblasts) cell line. The cell viability was measured by three tests: the MTT reduction, LDH release and Neutral Red uptake. The type of cell death was determined by photometric enzyme immunoassay. Moreover, the IL-1α and IL-6 concentration was determined by the sandwich-linked immunosorbent assay.

The present study showed that iron chloride decreases cells viability (confirmed by three cell viability tests) after 24 hours of incubation. The suggested mechanism of iron action in mouse embryo fibroblasts can be related to the iron interaction with cell membrane, subsequntly with mitochondria and, finally with lysosomes. Moreover, iron decreases concentration of mono- and oligonucleosomes in the cytoplasm and IL-6 concentration secreted into the cell culture supernatant. However, the concentration of IL-1α increases after incubation with iron chloride.
keywords:

iron, cytotoxicity, necrosis, cytokines

Termedia
About us Contact
Copyright notice Privacy policy Advertising policy Contact us
Quick links
Journals Books eBooks Events
© 2024 Termedia Sp. z o.o.
Developed by Bentus.