eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2012
vol. 37
 
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abstract:

Experimental immunology
The influence of sterilized and non-sterilized amniotic dressings on the proliferation of endothelial cells in vitro

Piotr Skopiński
,
Robert Zdanowski
,
Tomasz Grzela
,
Artur Kamiński
,
Sławomir Lewicki
,
Ewa Skopińska-Różewska
,
Wanda Stankiewicz

(Centr Eur J Immunol 2012; 37 (2): 114-118)
Online publish date: 2012/05/22
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The amnion is considered as a rich source of factors beneficial for tissue regeneration. However, some studies revealed, that amniotic membrane also displays some anti-angiogenic properties.

The aim of the study was to compare the effect of radiation-sterilized and non-sterilized amnion dressings, on the proliferation of HECa10 endothelial cells (EC) in vitro. The influence of tissue samples on EC proliferation was evaluated with the Alamar Blue (AB) reagent.

Results: Non-sterilized amnion tissue significantly stimulated (p < 0.05) and radiation-sterilized samples highly significantly suppressed (p < 0.001) EC proliferation, as compare to the control cultures.

No difference was seen between the control fluorescence values (cultures with 10% of FBS) and cultures with non-sterilized amnion tissue homogenate. Significantly lower EC proliferation was observed in cell cultures containing homogenate of radiation-sterilized amnion membrane (p < 0.001).
keywords:

biological dressings, amnion, radio-sterilization, endothelial cells, proliferation

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