eISSN: 1509-572x
ISSN: 1641-4640
Folia Neuropathologica
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SCImago Journal & Country Rank
2/2019
vol. 57
 
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abstract:
Original paper

Astragaloside IV protects neurons from microglia-mediated cell damage through promoting microglia polarization

Jingwen Yu
,
Minfang Guo
,
Yanhua Li
,
Huiyu Zhang
,
Zhi Chai
,
Qing Wang
,
Yuqing Yan
,
Jiezhong Yu
,
Chunyun Liu
,
Guangxian Zhang
,
Ma Cungen

Folia Neuropathol 2019; 57 (2):170-181
Online publish date: 2019/06/28
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Astragaloside IV (AST-IV) is a major active ingredient of astragalus, with a neuroprotective effect. The current study is aimed to investigate the impact of AST-IV on the M1/M2 microglial activation in response to lipopolysaccharide (LPS) stimulation, how AST-IV attenuated microglia-mediated neuronal damage, and the molecular mechanisms underlying AST-IV’s protection of neurons against microglia-mediated neuronal damage. Our results showed that AST-IV partially protected microglia from death evoked by LPS and downregulated the release of pro-inflammatory (M1) mediators including interleukin (IL)-1β, IL-6, tumour necrosis factor α (TNF-α) and nitric oxide, as well as the expression of Toll-like receptors 4 (TLR4), MyD88, and nuclear factor κB (NF-κB) of these cells. In contrast, AST-IV elevated the production of anti-inflammatory cytokine IL-10 and expression of arginase 1, an M2 marker of microglia, whose conditioned medium promoted PC12 neurons survival. These results indicate that AST-IV exerts an anti-inflammatory effect on microglia, possibly through inhibiting TLR4/NF-κB signalling pathways, and protects neurons from microglia-mediated cell death through conversion of microglia from inflammatory M1 to an anti-inflammatory M2 phenotype.
keywords:

astragaloside IV, neuroinflammatory, neurons, microglia polarization, TLR4 intracellular pathways

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