Batatasin-III protects cerebral vascular endothelium 
by regulating mitochondrial function and production of nitric oxide

Zhuyan Huang; Ling Li; Shanshan Huang; Yafeng Zhang; Qing Zhang; Zhimei Li; Yue Cao; Wenjuan Yu; Shuhua Tong; Qiang Zhang

doi:10.5114/fn.2025.154412

Abstract

3/2025 vol. 63

Original paper

Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide

Zhuyan Huang ¹

,

Ling Li ¹

,

Shanshan Huang ¹

,

Yafeng Zhang ¹

,

Qing Zhang ¹

,

Zhimei Li ¹

,

Yue Cao ¹

,

Wenjuan Yu ¹

,

Shuhua Tong ¹

,

Qiang Zhang ¹

Department of Neurology Nursing, Tongji Hospital of Huazhong University of Science and Technology, Wuhan, Hubei Province, China, 430000, China

Folia Neuropathol 2025; 63 (3): 248-255

DOI: https://doi.org/10.5114/fn.2025.154412

Online publish date: 2025/09/30

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AMA

Huang Z, Li L, Huang S, et al. Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide. Folia Neuropathologica. 2025;63(3):248-255. doi:10.5114/fn.2025.154412.

APA

Huang, Z., Li, L., Huang, S., Zhang, Y., Zhang, Q., & Li, Z. et al. (2025). Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide. Folia Neuropathologica, 63(3), 248-255. https://doi.org/10.5114/fn.2025.154412

Chicago

Huang, Zhuyan, Ling Li, Shanshan Huang, Yafeng Zhang, Qing Zhang, Zhimei Li, and Yue Cao et al. 2025. "Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide". Folia Neuropathologica 63 (3): 248-255. doi:10.5114/fn.2025.154412.

Harvard

Huang, Z., Li, L., Huang, S., Zhang, Y., Zhang, Q., Li, Z., Cao, Y., Yu, W., Tong, S., and Zhang, Q. (2025). Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide. Folia Neuropathologica, 63(3), pp.248-255. https://doi.org/10.5114/fn.2025.154412

MLA

Huang, Zhuyan et al. "Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide." Folia Neuropathologica, vol. 63, no. 3, 2025, pp. 248-255. doi:10.5114/fn.2025.154412.

Vancouver

Huang Z, Li L, Huang S, Zhang Y, Zhang Q, Li Z et al. Batatasin-III protects cerebral vascular endothelium by regulating mitochondrial function and production of nitric oxide. Folia Neuropathologica. 2025;63(3):248-255. doi:10.5114/fn.2025.154412.

This study assessed the effect of batatasin-III on cerebral microvascular endothelial cell (EC) injury. Endothelial cell injury was induced in isolated cells by oxygenglucose deprivation/reperfusion (OGD/R) conditions. During 4 h of OGD insult, cells were treated with batatasin-III at different concentrations, and an MTT assay was performed to estimate cellular viability. Nitric oxide, mitochondrial membrane potential (MMP), and release of lactate dehydrogenase (LDH) were determined in ECs exposed to OGD/R. Cerebral ischemia was performed to induce brain injury to assesses the effect of batatasin-III. There was improvement in the viability of cell and reduction of release of LDH in batatasin-III-treated, OGD/R-induced EC injury. Treatment with batatasin-III ameliorates the altered integrity of mitochondrial membrane and expression of apoptotic proteins in OGD/R injured ECs. Apoptosis of neuronal cells and infarct size were reduced in batatasin-III-treated middle cerebral artery occlusion (MCAO) rats, and pathological changes were reversed. Based on study data, we concluded that cerebral injury was attenuated by batatasin-III treatment by reducing oxidative stress and cellular apoptosis.

Keywords

batatasin-III, endothelial cell, neuron, oxygen, glucose, reperfusion

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