eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3-4/2006
vol. 31
 
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abstract:

Clinical immunology
Immunomodulatory effect of recombinant human procalcitonin on mitogenic activity of lymphocytes

Mária Bucová
,
Roman Záhorec
,
Milan Buc

Centr Eur J Immunol 2006; 31 (3-4): 87-93
Online publish date: 2007/01/16
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Procalcitonin (PCT) is an excellent inflammatory marker for early diagnosis of sepsis. However, little is known about its immunological functions.

Objective: The aim of our work was to determine the effect of recombinant human (rh) PCT on blastic transformation of lymphocytes.

Methods: Peripheral blood lymphocytes from 9 healthy volunteers were isolated on Ficoll-Metrizoat gradient and cultivated with polyclonal mitogens phytohemaglutinin (PHA), concanavalin A (Con A) and lipopolysaccharide (LPS) with or without rhPCT. RhPCT was added to the cultures in four different final concentrations.

Results: 1. RhPCT significantly inhibited the blastic transformation of PHA activated T-lymphocytes
(by 14-26%) as well as the simultaneous activation of T cells with rhPCT and PHA (21%). 2. RhPCT significantly decreased the ability of T cells stimulated by Con A either simultaneously (13-19%)
or subsequently (21-41%). 3. RhPCT increased the activity of unstimulated autologous lymphocytes.

Conclusion: Our preliminary results indicate that rhPCT suppressed the activity of both PHA and Con A stimulated T-cells, however it increased the activity of unstimulated lymphocytes. The results indicate that in vivo production of PCT in septic patients might contribute to a suppression of the hyperactivated specific cellular immunity.
keywords:

immunity, lymphocytes, mitogens, procalcitonin, sepsis

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