eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
Current issue Archive Manuscripts accepted About the journal Supplements Editorial board Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
3/2022
vol. 73
 
Share:
Share:
Original paper

Clinical significance and effect of PRR 11 up-regulation on the malignancy of osteosarcoma

Rui Tan
1
,
Chunbing Zhao
2
,
Yangcheng Lu
1
,
Lei Chen
1

1.
Orthopedic Center, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China
2.
Department of Pharmacy, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, Xinjiang, China Rui Tan and Chunbing Zhao contributed equally to this work.
Pol J Pathol 2022; 73 (3): 264-276
Online publish date: 2023/01/10
Article file
Get citation
 
PlumX metrics:
 

Introduction

Osteosarcoma is a malignant tumor of the skeletal system, which is derived from mesenchymal cells, and has the highest morbidity and mortality among all primary bone tumors [1]. Currently accepted treatments for osteosarcoma include surgery, chemotherapy, and radiation therapy [2]. With the continuous improvement in the treatment methods, the five-year survival rate of patients with osteosarcoma has reached 60–70%. However, the overall five-year survival of patients with recurrent or metastatic osteosarcoma has undergone little change and is approximately 20% [3]. In recent decades, with the maturation and wide application of second- generation high-throughput sequencing technology, extensive research on tumor tissue and cell sequencing has provided reliable data for gene-targeted therapy. Oncology research has thus been ushered into the era of “data explosion”. Bioinformatic methods have been used to screen tumor mRNA [4], circRNAs [5], lncRNA [6], the circRNA-miRNA-mRNA network [7], tumor microenvironment genes [8], and N6-methyladenosine [9]. Although there have been several studies, the clinical outcomes of patients with osteosarcoma have not significantly improved; therefore, the identification of new effective treatments is critical.
PRR11, a newly identified gene with oncogenic potential, is a proline-rich protein encoded by the PRR11 gene located in the amplified region of 17q22 [10]. It has been found that PRR11 participates in cell cycle regulation by regulating cell cycle transcription factor-related binding sites, thus affecting cell proliferation, apoptosis, cell cycle progression, and carcinogenesis [11]. It was confirmed that PRR11 being expressed highly by various malignant tumors has a significant correlation with prognosis. However, the role of PRR11 in osteosarcoma has only been reported in a few studies [12]. At present, the treatment of osteosarcoma has hit a bottleneck. In this study, we used bioinformatics to mine public database data for analyzing the expression of PRR11 in osteosarcoma; further, we studied the clinicopathological characteristics of PRR11 and its relationship with osteosarcoma prognosis. Simultaneously, we verified the malignant biological behavior of PRR11 in osteosarcoma via in vitro cell experiments, which may help in determining a targeted therapy for osteosarcoma.

Material and methods

Public database data retrieval
In the Gene Expression Profiling Interactive Analysis (GEPIA) database, we searched the expression of the PRR11 gene in various normal tissues and cancer tissues. In the Cancer Cell Line Encyclopedia (CCLE) database, we downloaded the PRR11 transcriptome data and analyzed the differential expression level of PRR11 between osteosarcoma and other cancer cell lines. The whole transcriptome sequencing data and clinical data of PRR11 in osteosarcoma tissues from the The Cancer Genome Atlas (TCGA) database were downloaded using UCSC Xena.
Data processing
We used the “ggplot2” package in the R software (4.1.2) to process the PRR11 transcriptome data downloaded in CCLE. Using the “DESeq2” package, the samples were chopped up into a high and a low expression group based on the median of PRR11 expression, and the whole transcriptome sequencing data of the PRR11 gene in osteosarcoma tissues in TCGA were analyzed. The clinical data were analyzed by univariate Cox regression and Kaplan-Meier survival curve operating with the “survival” and “survminer” packages.
Gene set enrichment analysis and protein-protein interaction network analysis
The gene set enrichment analysis software was used to analyze the gene expression matrix processing of samples of 256 patients with osteosarcoma obtained from the TCGA database, and pathways with a p-value < 0.01 and false discovery rate < 0.25 were screened out. Using STRING to construct a protein-protein interaction (PPI) protein network with PRR11 protein as the core, the biological functions of these proteins were analyzed. It is significant for elucidating the mechanism by which PRR11 adjusts the occurrence and progression of osteosarcoma. Retrieval conditions: 1) Protein Name: PRR11; 2) Orga­nism: Homo sapiens.
Cells and main reagents
The osteosarcoma cell line MG63 was sourced from the Shanghai Cell Bank, Chinese Academy of Sciences. PRR11 rabbit anti-human polyclonal antibody (Ab237526) was bought from the Abcam Company, USA. Trizol was bought from Ambion, USA. Reverse transcription kit PrimeScript II RTase and Recombinant RNase Inhibitor were bought from TAKARA. Goat anti-mouse IgG, goat anti-rabbit, and DAB chromogenic kits were bought from OriGene, USA. SYBR FAST qPCR Master Mix was bought from KAPABiosystems. Lipofectamine 2000 was bought from Invitrogen, USA. MEM, Opti-MEM, and fetal bovine serum were bought from Gibco, USA. PBS, 0.25% trypsin, and the cell counting kit were bought from Beijing solarbio. Transwell chambers were bought from Corning, USA.
Cell culture and transfection
MG63 cells were cultured in 10% MEM in an incubator under culture conditions of 5% CO2, 10% humidity, and a temperature of 37°C. Transfection was performed using Lipofectamine 2000 cationic lipid transfection reagent based on the manufacturer’s instructions. MG-63 cells in the log phase were taken and seeded in a 6-well plate at a density of 5×105 cells/well for cell culture. Lipofectamine 2000 was used to transfect the empty control group and the PRR11 high expression group. After four hours, the medium containing the transfection reagent was replaced with the normal medium. The transfected cells were cultured for 48 hours at 37°C and in a 5% CO2 incubator, which will be used for subsequent experiments.
Quantitative real-time polymerase chain reaction
Trizol reagent was used to extract total RNA from the cells based on the kit instructions. cDNA was obtained by the reverse transcription kit, and the amplification of the target gene was obtained by the fluorescent quantitative PCR kit. The relative expression level of RNA was expressed by 2 -ΔΔCt. The experiments were repeated three times. See Table I for the primer sequences.
Total protein extraction and western-blot detection
The total protein was extracted with RIPA lysate, and quantitatively evaluated by the BCA protein assay kit. Subsequently, 20 µg of protein solution per well was loaded for SDS-PAGE electrophoresis, and the proteins were transferred to a PVDF membrane, soaked in the primary antibody incubation solution, and incubated overnight at 4°C. The next day, the membrane was washed with PBST, then incubated with the secondary antibody for 1 h at room temperature, washed with PBST, followed by detection of light emission by control label. The membrane was placed in an automatic chemiluminescence analyzer and the gray value of the relevant bands was read by TANON GIS software. The data were independently replicated three times.
Cell proliferation assay
The cells that were stored in a 96-well plate were cultured in an incubator at 37°C. Ten µl of cell counting kit-8 solution was added at culture time intervals of 24, 48, and 72 hours, and after 1 hour incubation. We used a microplate reader to detect the absorbance value at 450 nm and draw the proliferation curve. The experiment was repeated three times.
Apoptosis detection
For apoptosis detection, 1×106 cells were digested into a cell suspension, then Annexin V-PE and 7-AAD were added, followed by incubation in the dark for 30 minutes at 4°C. On detection by flow cytometry, strong 7-AAD indicates dead cells, weak 7-AAD indicates apoptotic cells, and no 7-AAD indicates normal viable cells. The NovoExpress analysis software was used for calculating the number of apoptotic cells. The data were independently replicated three times.
Invasion assay
A 0.5 ml cell suspension containing a concentration of 1×105 cells/ml was inoculated into each transwell. Subsequently, 0.75 ml of 10% fetal bovine serum medium was added to the lower 24-well plate and incubated at 37°C for 24 hours. We stained the cells with crystal violet solution and observed the number of cells using a microscope.
Wound healing assay
A total of 1×10 6 cells were added to each 6-well plate. When the bottom of the 6-well plate was covered with cells, a sterilized pipette tip was used to draw three lines, which were parallel to the central axis. Photographs of the scratches were taken at 0 and 24 hours, and ImageJ software was used to measure the width of the scratches.
Statistical analysis
The data were analyzed by SPSS 22.0 statistical software, and measurement data were expressed as means ± standard deviation. The differences among multiple groups were compared by ANOVA. The statistical significance was set at p < 0.05.
Ethical statement
This study is mainly aimed at cell experiments and bioinformatics database research, and does not involve animal experiments and patient studies. This experiment has been solicited for ethical review by the Ethics Committee of the First Affiliated Hospital of Shihezi University School of Medicine (batch number: KJ2022-138-01). This study was exempt from ethical approval.
Data availability statement
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Results

PRR11 expression in malignant tumors and tumor cells
In the GEPIA database, it was revealed that PRR11 was higher in most malignant tumors compared to normal tissues (Fig. 1A), and was higher in sarcomas, with the difference being significant (p < 0.05) (Fig. 1B). In order to understand the expression of PRR11 in osteosarcoma cells, data from the CCLE database were analyzed and the results showed that the expression of PRR11 in osteosarcoma cells ranked eighth among the 33 malignant tumor cell lines included in it (Fig. 1C). There were also differences in the expression of PRR11 among the osteosarcoma cell lines, with higher PRR11 expression in U2OS cells and lower expression in MG63 cells (Fig. 1D).
Survival analysis and clinical characteristics of PRR11 in osteosarcoma
The original data of patients with osteosarcoma were obtained from the TCGA database. After screening, there were 265 osteosarcoma samples with complete clinical information. The median expression of PRR11 was used to classify the samples into a high and a low expression group. The Kaplan-Meier survival curve showed that the overall survival and disease-specific survival of patients with osteosarcoma in the low PRR11 expression group were significantly higher than those of the high-expression group, and the difference was significant (Fig. 2A,B). However, there was no significant difference in the disease-free survival and progression-free survival in patients with osteosarcoma (Fig. 2C,D). To understand the independent prognostic indicators for survival in osteosarcoma, univariate regression analysis was performed on the clinical characteristics, and the results showed that PRR11 expression, age, tumor length, tumor depth, tumor width, tumor multifocality, tumor recurrence, and metastasis were the risk factors affecting the prognosis. However, radiotherapy, tumor necrosis percentage, and gender did not affect prognosis (Table II).
PRR11 gene set enrichment analysis pathway enrichment analysis in osteosarcoma
To study the enrichment pathways related to PRR11 in osteosarcoma, we used the gene set enrichment analysis (GSEA) software to perform pathway enrichment analysis on genes associated with PRR11 expression in osteosarcoma using the transcriptome data downloaded from the TCGA database. The results showed six pathways with significant significance (p < 0.01) (Table III). PRR11 is mainly involved in ubiquitin-mediated proteolysis, the cell cycle, and other pathways in osteosarcoma (Fig. 3).
Analysis of the protein interactions of PRR11
To explore the upstream and downstream relationships of PRR11 in osteosarcoma, the STRING was used, which showed that a total of 11 interaction nodes with p = 2.53e–09 were enriched. The genes with an interaction score > 0.400 were CDC20, CCNA2, SKA2, GTSE1, TOP2A, CKAP2L, CKAP2, DLGAP5, AXIN2, and KIF11 (Fig. 4). These genes are mainly involved in cell cycle regulation, including sister chromosome segregation, and nuclear separation (Table IV).
Transfection of MG-63 cells with PRR11
The CCLE database and results of previous studies showed that the expression level of PRR11 was lower in MG-63 cells. Therefore, we selected the MG-63 cell line and transfected it with the high expression plasmid and the empty plasmid, respectively. Quantitative real-time polymerase chain reaction and Western blot showed that high-expression transfection effectively increased the level of PRR11 in MG-63 cells compared with the control group (p < 0.05, **p < 0.01) (Fig. 5A,B).
PRR11 promotes proliferation of osteosarcoma MG-63 cells and inhibits apoptosis
Cell counting kit-8 assay revealed that after 24, 48, and 72 hours of transfection, the proliferation of the high expression group was significantly enhanced compared to the empty control group and blank control group (Fig. 6A). The flow cytometry revealed that compared to the empty group and the blank control group, the overall apoptosis rate of the PRR11 high expression group was reduced (Fig. 6B) (p < 0.05, **p < 0.01).
PRR11 promotes invasion and migration of osteosarcoma cells
Next, we assessed the effect of PRR11 on migration and invasion of MG-63 cells. The results of the scratch test revealed that 24 hours after transfection, the migration rate of the high expression group was markedly increased compared to the empty control group and the blank control group (Fig. 7A). The results of the Transwell assay showed that 24 hours after transfection, cell invasion of MG-63 cells in the high expression group was increased compared to the empty control group and the blank control group (Fig. 7B) (*p < 0.05, **p < 0.01).

Discussion

Cell division is necessary for tissue growth and development, and mutations and deletions involving cell cycle regulation may lead to erroneous cell division and chromosome segregation, resulting in cell cycle disorders. Eventually, this may lead to serious diseases, such as tumorigenesis [13, 14]. There is increasing evidence that the inactivation of tumor suppressor genes or activation of proto-oncogenes can affect the cell cycle and lead to tumor initiation and progression [15, 16].
The tumor-related gene PRR11 is a newly discovered gene situated on chromosome 17q22, consisting of 10 exons and 9 introns, and the translation initiation codon is situated in the second exon, while the stop codon is situated in the last exon [17]. Many researchers have found that PRR11 is highly expressed in cancers such as colorectal, liver, breast, and ovarian cancer, and is closely related to poor prognosis [18–21]. However, only limited studies have reported the role of PRR11 in osteosarcoma. Song et al. found that miR-211-5p regulates PRR11 to promote apoptosis and inhibit the migration of osteosarcoma cells [12]. Our group previously found that the expression of PRR11 was elevated in osteosarcoma, and the proliferation, invasion, and migration of osteosarcoma cells were inhibited by downregulation of PRR11 [22]. In the present study, we used the GEPIA and CCLE databases and found that compared with normal tissues, the expression level of PRR11 is high in the vast majority of malignant tumors, including sarcomas. Also, the expression of PRR11 in osteosarcoma cells is high in a variety of malignant cell lines. The expression of PRR11 was the highest in U2OS cells, which was accordant with the finding of previous cell experiments.
Using bioinformatics methods, the relationship between genes and tumor prognosis can be analyzed quickly and cost-effectively. At the same time, data sets with multiple large sample sets are more reliable than single-sample studies. Hau et al. screened many differentially expressed pancreatic cancer genes including PRR11 in TCGA, and the high expression of PRR11 significantly shortened the five-year overall survival of pancreatic cancer and was an independent risk factor [23]. Wang et al. found that the expression level of PRR11 was related to the clinical stage, and T and N of TNM staging. Univariate and multivariate analysis showed that PRR11 was an independent prognostic factor for squamous cell carcinoma of the tongue [24]. In accordance with the TCGA database, the present study found that PRR11 had a significant effect on the survival rate of osteosarcoma, and the survival rate of the low expression group was significantly higher than that of the high expression group. In addition, in patients with osteosarcoma, age, tumor length, tumor depth, tumor width, tumor multifocality, tumor recurrence, and metastasis were the risk factors affecting prognosis. This is consistent with the above research results and provides evidence for the involvement of the PRR11 gene in the progression of osteosarcoma.
Regarding the mechanism by which PRR11 pro­motes tumor progression, many bioinformatics studies have shown that PRR11 is mainly involved in regulation of the cell cycle, M phase, and mitotic cell cycle, and in various tumor signaling pathways [25–27]. In addition, a large number of cell experiments have also shown that PRR11 affects cell migration, proliferation, and invasion in a variety of tumors. Zhou et al. found that silencing of PRR11 inhibited the migration and proliferation of esophageal cancer cells and significantly up-regulated the expression of E-cadherin [28]. Wang et al. reported that overexpression of PRR11 significantly increased the invasion and proliferation of tongue squamous cell carcinoma, while PRR11 knockout had the opposite effect [29]. In the present study, through the GSEA pathway enrichment analysis, it was found that PRR11 was mainly enriched in pathways correlated with the cell cycle and cell division in osteosarcoma. Further PPI network analysis revealed that the proteins that bind to PRR11 are mainly CDC20, CCNA2, and SKA2, which predominantly participate in cell cycle regulation. We further verified the influence of PRR11 on osteosarcoma cells through cell experiments. After the up-regulation of PRR11 in MG-63 cells, the migration, invasion, and proliferation abilities of osteosarcoma cells were enhanced, and apoptosis was weakened. This was consistent with the results of the above studies.
This study has some limitations. The molecular mechanism of PRR11 regulating the cell cycle of osteosarcoma is still unclear. This research group will further study this at a later stage.

Conclusions

In summary, this study demonstrated the high expression of PRR11 in a variety of malignant tumors via bioinformatics methods; moreover, the high expression is significantly correlated with prognosis. Gene set enrichment analysis and PPI network analysis revealed that PRR11 is mainly involved in regulation of the cell cycle and other pathways in osteosarcoma. Up-regulation of PRR11 promotes migration, invasion, and proliferation of osteosarcoma cells and inhibits apoptosis. These data strongly suggest that PRR11 may be a predictor of survival in patients with osteosarcoma and a therapeutic target.

Acknowledgments

The authors thank GEPIA, CCLE, and TCGA for their contributions to human tumor research and the establishment of open databases. We thank everyone who participated in this study.

Funding

This work was supported in part by Shihezi University scientific research project (ZZZC201818A), the National Natural Science Foundation of China (81860398), and the Science and Technology Program of the Xinjiang Production and Construction Corps (2022ZD034). The authors declare no conflicts of interest.

References

1. Ritter J, Bielack SS. Osteosarcoma. Ann Oncol 2010; 21: vii320-5.
2. Zhao X, Wu Q, Gong X, Liu J, Ma Y. Osteosarcoma: a review of current and future therapeutic approaches. Biomed Eng Online 2021; 20: 24.
3. Tong CJ, Deng QC, Ou DJ, Long X, Liu H, Huang K. LncRNA RUSC1-AS1 promotes osteosarcoma progression through regulating the miR-340-5p and PI3K/AKT pathway. Aging (Albany NY) 2021; 13: 20116-20130.
4. Han YL, Luo D, Habaxi K, et al. COL5A2 Inhibits the TGF-beta and Wnt/beta-Catenin Signaling Pathways to Inhibit the Invasion and Metastasis of Osteosarcoma. Front Oncol 2022; 12: 813809.
5. Lin F, Wang X, Zhao X, Ren M, Wang Q, Wang J. Circ_0001174 facilitates osteosarcoma cell proliferation, migration, and invasion by targeting the miR-186-5p/MACC1 axis. J Orthop Surg Res 2022; 17: 159.
6. Tang J, Zhu Z, Dong S, et al. Long non-coding RNA long intergenic non-coding 00641 mediates cell progression with stimulating cisplatin-resistance in osteosarcoma cells via microRNA-320d/myeloid cell leukemia-1 axis. Bioengineered 2022; 13: 7238-7252.
7. Wu T, Wei B, Lin H, et al. Integral analyses of competing endogenous RNA mechanisms and DNA methylation reveal regulatory mechanisms in osteosarcoma. Front Cell Dev Biol 2021; 9: 763347.
8. Shi D, Mu S, Pu F, et al. Integrative analysis of immune-related multi-omics profiles identifies distinct prognosis and tumor microenvironment patterns in osteosarcoma. Mol Oncol 2021.
9. Wei K, Gao Y, Wang B, Qu YX. Methylation recognition protein YTH N6-methyladenosine RNA binding protein 1 (YTHDF1) regulates the proliferation, migration and invasion of osteosarcoma by regulating m6A level of CCR4-NOT transcription complex subunit 7 (CNOT7). Bioengineered 2022; 13: 5236-5250.
10. Wang Y, Weng H, Zhang Y, et al. The PRR11-SKA2 bidirectional transcription unit is negatively regulated by p53 through NF-Y in lung cancer cells. Int J Mol Sci 2017; 18: 534.
11. Hu H, Song Z, Yao Q, et al. Proline-rich protein 11 regulates self-renewal and tumorigenicity of gastric cancer stem cells. Cell Physiol Biochem 2018; 47: 1721-1728.
12. Song D, Yang K, Wang W, Tian R, Wang H, Wang K. MicroRNA-211-5p promotes apoptosis and inhibits the migration of osteosarcoma cells by targeting proline-rich protein PRR11. Biochem Cell Biol 2020; 98: 258-266.
13. Qin A, Reddy HG, Weinberg FD, Kalemkerian GP. Cyclin- dependent kinase inhibitors for the treatment of lung cancer. Expert Opin Pharmacother 2020; 21: 941-952.
14. Wenzel ES, Singh ATK. Cell-cycle checkpoints and aneuploidy on the path to cancer. In Vivo 2018; 32: 1-5.
15. Yin L, Yan J, Wang Y, Sun Q. TIGD1, a gene of unknown function, involves cell-cycle progression and correlates with poor prognosis in human cancer. J Cell Biochem 2019; 120: 9758-9767.
16. Zhang H, Zhang X, Li X, et al. Effect of CCNB1 silencing on cell cycle, senescence, and apoptosis through the p53 signaling pathway in pancreatic cancer. J Cell Physiol 2018; 234: 619-631.
17. Kimura K, Wakamatsu A, Suzuki Y, et al. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. Genome Res 2006; 16: 55-65.
18. Zhong F, Liu S, Hu D, Chen L. LncRNA AC099850.3 promotes hepatocellular carcinoma proliferation and invasion through PRR11/PI3K/AKT axis and is associated with patients prognosis. J Cancer 2022; 13: 1048-1060.
19. Qiao W, Wang H, Zhang X, Luo K. Proline-rich protein 11 silencing inhibits hepatocellular carcinoma growth and epithelial-mesenchymal transition through beta-catenin signaling. Gene 2019; 681: 7-14.
20. Wang Y, Zhang C, Mai L, Niu Y, Wang Y, Bu Y. PRR11 and SKA2 gene pair is overexpressed and regulated by p53 in breast cancer. BMB Rep 2019; 52: 157-162.
21. Zhan Y, Wu X, Zheng G, et al. Proline-rich protein 11 overexpression is associated with a more aggressive phenotype and poor overall survival in ovarian cancer patients. World J Surg Oncol 2020; 18: 318.
22. Li K, Yu H, Zhao C, Li J, Tan R, Chen L. Down-regulation of PRR11 affects the proliferation, migration and invasion of osteosarcoma by inhibiting the Wnt/beta-catenin pathway. J Cancer 2021; 12: 6656-6664.
23. Hau OS, Wahlin S, Cervin S, et al. PRR11 unveiled as a top candidate biomarker within the RBM3-regulated transcriptome in pancreatic cancer. J Pathol Clin Res 2022; 8: 65-77.
24. Wang C, Yu L, Hu F, et al. Upregulation of proline rich 11 is an independent unfavorable prognostic factor for survival of tongue squamous cell carcinoma patients. Oncol Lett 2017; 14: 4527-4534.
25. Chen N, Zhang G, Fu J, Wu Q. Identification of key modules and hub genes involved in esophageal squamous cell carcinoma tumorigenesis using WCGNA. Cancer Control 2020; 27: 1073274820978817.
26. Gu Y, Li J, Guo D, et al. Identification of 13 key genes correlated with progression and prognosis in hepatocellular carcinoma by weighted gene co-expression network analysis. Front Genet 2020; 11: 153.
27. Sun Y, Xu D, Zhang C, et al. HEDGEHOG/GLI modulates the PRR11-SKA2 bidirectional transcription unit in lung squamous cell carcinomas. Genes (Basel) 2021; 12: 120.
28. Zhou L, Deng ZZ, Li HY, et al. Overexpression of PRR11 promotes tumorigenic capability and is associated with progression in esophageal squamous cell carcinoma. Onco Targets Ther 2019; 12: 2677-2693.
29. Wang C, Yu L, Ren X, et al. The oncogenic potential of PRR11 gene in tongue squamous cell carcinoma cells. J Cancer 2019; 10: 2541-2551.
Copyright: © 2023 Polish Association of Pathologists and the Polish Branch of the International Academy of Pathology This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) License (http://creativecommons.org/licenses/by-nc-sa/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material, provided the original work is properly cited and states its license.
Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.