eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
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4/2018
vol. 69
 
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abstract:
Original paper

Comet assay is not useful to predict normal tissue response after radiochemotherapy in cervical and larynx cancer patients

Agnieszka Adamczyk, Beata Biesaga, Małgorzata Klimek, Anna Mucha-Małecka

Pol J Pathol 2018; 69 (4): 410-421
Online publish date: 2019/01/31
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Normal tissues reactions after radiotherapy vary considerably even between patients receiving the same treatment. The ability to predict the differences in radiosensitivity before radiotherapy would have important implication.

Patients with squamous cell carcinoma of the: (i) cervix (38 patients) and (ii) larynx (19 patients) were studied. Control group consisted of 9 healthy women. To assess individual radiosensitivity/chemoradiosensitivity alkaline version of comet assay was performed using isolated peripheral blood lymphocytes from cancer patients and healthy donors. The level of endogenous (0Gy), initial (immediately after 6Gy irradiation) and residual (after irradiation and 1h of repair) DNA damage was investigated.

The mean value of endogenous damage was similar in control and cervical cancer (CCU) groups and significantly lower than in larynx cancer patients. Cancer patients showed slower DNA repair. For CCU and larynx patients, comet assay parameters were not helpful for unequivocal prediction of appearance of acute and late radiation reaction effects.

Comet assay seems to be unable to predict normal tissue reaction after radiochemotherapy. Therefore, there is still need for developing predictive assays, however, due to complicated mechanism of chemoradiosensitivity, only assays assessing not one but many molecular pathways might gives us reliable score.
keywords:

comet assay, chemoradiosensitivity, cervix cancer patients, larynx cancer patients

references:
Turesson I, Nyman J, Holmberg E, Odén A. Prognostic factors for acute and late skin reactions in radiotherapy patients. Int
J Radiat Oncol Biol Phys 1996; 36: 1065-1075.
Fernet M, Hall J. Predictive markers for normal tissue reactions: fantasy or reality? Cancer Radiother 2008; 12: 614-618.
Andreassen CN, Alsner J. Genetic variants and normal tissue toxicity after radiotherapy: a systematic review. Radiother
Oncol 2009; 92: 299-309.
Andreassen CN. Can risk of radiotherapy-induced normal tissue complications be predicted from genetic profiles? Acta Oncol
2005; 44: 801-815.
Andreassen CN, Alsner J, Overgaard J. Does variability in normal tissue reactions after radiotherapy have a genetic basis-where and how to look for it? Radiother Oncol 2002; 64: 131-140.
Barnett GC, West CM, Dunning AM, et al. Normal tissue reactions to radiotherapy: towards tailoring treatment dose by genotype. Nat Rev Cancer 2009; 9: 134-142.
Budach W, Classen J, Belka C, Bamberg M. Clinical impact of predictive assays for acute and late radiation morbidity. Strahlenther Onkol 1998; 174 Suppl 3: 20-24.
Azqueta A, Slyskova J, Langie SA, et al. Comet assay to measure DNA repair: approach and applications. Front Genet 2014; 5: 288.
Brendler-Schwaab S, Hartmann A, Pfuhler S, Speit G. The in vivo comet assay: use and status in genotoxicity testing. Mutagenesis 2005; 20: 245-254.
Collins A, Koppen G, Valdiglesias V, et al. The comet assay as a tool for human biomonitoring studies: the ComNet project. Mutat Res Rev Mutat Res 2014; 759: 27-39.
Fikrová P, Stětina R, Hronek M, et al. Application of the comet assay method in clinical studies. Wien Klin Wochenschr 2011; 123: 693-699.
McKenna DJ, McKeown SR, McKelvey-Martin VJ. Potential use of the comet assay in the clinical management of cancer. Mutagenesis 2008; 23: 183-190.
Wang WD, Chen ZT, Li DZ, et al. Correlation between DNA repair capacity in lymphocytes and acute side effects to skin during radiotherapy in nasopharyngeal cancer patients. Clin Cancer Res 2005; 11: 5140-5145.
Gabelova A, Farkasova T, Gurska S, et al. Radiosensitivity of peripheral blood lymphocytes from healthy donors and cervical cancer patients; the correspondence of in vitro data with the clinical outcome. Neoplasma 2008; 55: 182-191.
Padjas A, Kedzierawski P, Florek A, et al. Comparative analysis of three functional predictive assays in lymphocytes of patients with breast and gynaecological cancer treated by radiotherapy. J Contemp Brachytherapy 2012; 4: 219-226.
Cortés-Gutiérrez EI, Hernández-Garza F, García-Pérez JO, et al. Evaluation of DNA single and double strand breaks in women with cervical neoplasia based on alkaline and neutral comet assay techniques. J Biomed Biotechnol 2012; 2012: 385245.
Buchynska L, Brieieva O, Glushchenko N, et al. DNA repair deficiency in peripheral blood lymphocytes of endometrial cancer patients with a family history of cancer. BMC Cancer 2014; 14: 765.
Kopjar N, Milas I, Garaj-Vrhovac V, Gamulin M. Alkaline comet assay study with breast cancer patients: evaluation of baseline and chemotherapy-induced DNA damage in non-target cells. Clin Exp Med 2006; 6: 177-190.
Rajaee-Behbahani N, Schmezer P, Risch A, et al. Altered DNA repair capacity and bleomycin sensitivity as risk markers for non-small cell lung cancer. Int J Cancer 2001; 95: 86-91.
Nadin SB, Vargas-Roig LM, Drago G, et al. DNA damage and repair in peripheral blood lymphocytes from healthy individuals and cancer patients: a pilot study on the implications in the clinical response to chemotherapy. Cancer Lett 2006; 239: 84-97.
Twardella D, Popanda O, Helmbold I, et al. Personal characteristics, therapy modalities and individual DNA repair capacity as predictive factors of acute skin toxicity in an unselected cohort of breast cancer patients receiving radiotherapy. Radiother Oncol 2003; 69: 145-153.
Palyvoda O, Pogañska J, Wygoda A, Rzeszowska-Wolny J. DNA damage and repair in lymphocytes of normal individuals and cancer patients: studies by the comet assay and micronucleus tests. Acta Biochimica Polonica 2003; 50: 181-190.
Popanda O, Ebbeler R, Twardella D, et al. Radiation-induced DNA damage and repair in lymphocytes from breast cancer patients and their correlation with acute skin reactions to radiotherapy. Int J Radiat Oncol Biol Phys 2003; 55: 1216-1225.
Alapetite C, Thirion P, de la Rochefordière A, et al. Analysis by alkaline comet assay of cancer patients with severe reactions to radiotherapy: defective rejoining of radioinduced DNA strand breaks in lymphocytes of breast cancer patients. Int J Cancer 1999; 83: 83-90.
Rusin P, Olszewski J, Morawiec-Bajda A, et al. Comparative study of DNA damage and repair in head and neck cancer after radiation treatment. Cell Biol International 2009; 33: 357-363.
Müller WU, Bauch T, Stüben G, et al. Radiation sensitivity of lymphocytes from healthy individuals and cancer patients as measured by the comet assay. Radiat Environ Biophys 2001; 40: 83-89.
Oppitz U, Denzinger S, Nachtrab U, et al. Radiation-induced comet-formation in human skin fibroblasts from radiotherapy patients with different normal tissue reactions. Strahlenther Onkol 1999; 175: 341-346.
Oppitz U, Schulte S, Stopper H, et al. In vitro radiosensitivity measured in lymphocytes and fibroblasts by colony formation and comet assay: comparison with clinical acute reactions to radiotherapy in breast cancer patients. Int J Radiat Biol 2002; 78: 611-616.
Shahidi M, Mozdarani H, Bryant PE. Radiation sensitivity of leukocytes from healthy individuals and breast cancer patients as measured by the alkaline and neutral comet assay. Cancer Lett 2007; 257: 263-273.
Smith TR, Miller MS, Lohman KK, et al. DNA damage and breast cancer risk. Carcinogenesis 2003; 24: 883-889.
Sterpone S, Cornetta T, Padua L, et al. DNA repair capacity and acute radiotherapy adverse effects in Italian breast cancer patients. Mutat Res 2010; 684: 43-48.
Rzeszowska-Wolny J, Palyvoda O, Polanska J, et al. Relationships between acute reactions to radiotherapy in head and neck cancer patients and parameters of radiation-induced DNA damage and repair in their lymphocytes. Int J Radiat Biol 2008; 84: 635-642.
Nascimento PA, da Silva MA, Oliveira EM, et al. Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients. Braz J Med Biol Res 2001; 34: 165-176.
Djuzenova CS, Mühl B, Fehn M, et al. Radiosensitivity in breast cancer assessed by the Comet and micronucleus assays. Br J Cancer 2006; 94: 1194-1203.
Hartley JM, Spanswick VJ, Hartley JA. Measurement of DNA damage in individual cells using the Single Cell Gel Electrophoresis (Comet) assay. Methods Mol Biol 2011; 731: 309-320.
Chua ML, Rothkamm K. Biomarkers of radiation exposure: can they predict normal tissue radiosensitivity? Clin Oncol
(R Coll Radiol) 2013; 25: 610-616.
Andreassen CN, Schack LM, Laursen LV, Alsner J. Radiogenomics – current status, challenges and future directions. Cancer Lett 2016; 382: 127-136.
Roberson JD 2nd, Burnett OL 3rd, Robin N. Radiogenomics: towards a personalized radiation oncology. Curr Opin Pediatr 2016; 28: 713-717.
Liu JC, Shen WC, Shih TC, et al. The current progress and future prospects of personalized radiogenomic cancer study. Biomedicine (Taipei) 2015; 5: 11-18.
Kerns SL, Ostrer H, Rosenstein BS. Radiogenomics: using genetics to identify cancer patients at risk for development of adverse effects following radiotherapy. Cancer Discov 2014; 4: 155-165.
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