eISSN: 2299-0046
ISSN: 1642-395X
Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii
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4/2017
vol. 34
 
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abstract:
Original paper

Cutaneous leukocytoclastic vasculitis: the roleof lymphocytes and related immune markers

Thilo Gambichler
,
Magdalena A. Kulik
,
Marina Skrygan
,
Isabelle Rooms
,
Stefan Höxtermann

Adv Dermatol Allergol 2017; XXXIV (4): 299–305
Online publish date: 2017/08/01
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Introduction: Apart from neutrophils, other immune cells may play a significant pathogenetic role in cutaneous leukocytoclastic vasculitis (CLV).


Aim: To investigate lymphocytes and related immunological factors in patients with CLV requiring systemic glucocorticosteroid treatment.


Material and methods: Fourteen patients with severe idiopathic CLV were treated with systemic prednisolone in a tapered dose regimen. Ten healthy individuals served as controls. At baseline and post-treatment, we studied inducer/helper and suppressor/cytotoxic T lymphocytes, B lymphocytes, natural killer cells, CD4+CD25++CD127– cells, CD4+CD25+CD39+ cells and FOXP3, transforming growth factor 1 (TGF-1) and interleukin-10 (IL-10) mRNA levels in the blood using flow cytometry and real time polymerase chain reaction (RT-PCR), respectively. On immunohistochemistry, we studied CD4, CD8, granzyme B, TGF-1, and IL-10.


Results: Flow cytometry did not show significant differences. The RT-PCR revealed that TGF-1 mRNA expression was significantly higher after therapy when compared to baseline and controls. On immunohistology, baseline CLV lesions showed significantly more CD4+ lymphocytes than post-treated CLV and controls. CD8+ expression was significantly higher after therapy when compared to baseline and controls. Baseline granzyme B was significantly increased when compared to treated CLV and controls. The IL-10 expression of treated CLV was significantly increased when compared to baseline CLV and; baseline CLV IL-10 expression was significantly increased as compared to controls.


Conclusions: Circulating T regulatory cells do not play a significant role in the pathogenesis of CLV. T helper cells and granzyme B seem to be involved in the inflammatory cutaneous process of CLV. A resolution of CLV observed after glucocorticosteroid treatment may be mediated via up-regulation of TGF-1 and IL-10 in different compartments.
keywords:

cutaneous vasculitis, lymphocytes, cytokines

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