eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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1/2003
vol. 28
 
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abstract:

Effects of M. catarrhalis antigens on IFN-γ, IL-4 and IL-13 production by PBMC from children with atopic asthma or recurrent respiratory tract infections

Daria Augustyniak
,
Grażyna Majkowska-Skrobek
,
Maria Kraus-Filarska
,
Adam Jankowski

(Centr Eur J Immunol 2003; 28 (1): 6–13)
Online publish date: 2004/01/20
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Abstract

The goal of this study was to evaluate the in vitro Th1/Th2 cytokine response as well as proliferative response in M. catarrhalis antigen-stimulated PBMC from 14 atopic asthma children (AA) and 11 non-atopic children suffering from recurrent respiratory tract infections and bronchospastic symptoms (RRTI). Membrane (MA) and soluble (SA) antigens of M. catarrhalis induced PBMC to production of IFN-g but did not induce cells to secretion of IL-4 and IL-13. The production of IFN-γ after stimulation with both MA and SA was considerably higher than spontaneous ones within each group (AA and control,
P<0.001; RRTI, P<0.01). Furthermore, proliferative response of PBMC following SA stimulation between 4- and 7-day of culture showed significant increase contrary to the MA stimulation in analogous time. PBMC from RRTI group secreted significantly less IFN-γ as compared with controls (MA, P<0.05; SA, P<0.001). Release of IL-4 and IL-13 by PBMC did not differ between the three groups. Concluding, the comparable productions of IFN-γ during stimulation of PBMC with MA and SA suggest, that apart from the former also the latter may have a strong impact on the Th1-type cytokine response. We proposed also that reduced IFN-γ in RRTI group may be one of important factors in non-effective defence mechanism.
keywords:

Moraxella catarrhalis, IFN-γ, IL-4, IL-13, specific IgE, atopic asthma, recurrent respiratory tract infections, PBMC

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