eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Abstracting and indexing Subscription Contact Instructions for authors
SCImago Journal & Country Rank

2/2010
vol. 35
 
Share:
Share:
more
 
 
abstract:

Experimental immunology
Chromium chloride cytotoxicity and cytokines production in BALB/c cell line

Sylwia Terpiłowska
,
Andrzej Krzysztof Siwicki

Centr Eur J Immunol 2010; 35 (2): 58-62
Online publish date: 2010/05/10
View full text
Get citation
ENW
EndNote
BIB
JabRef, Mendeley
RIS
Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
 
The aim of this study was to examine the cytotoxic effect of the chromium chloride on mouse fibroblasts. The experiments were performed on the BALB/c 3T3 (mouse BALB/c embryo fibroblasts) cell line. The cell viability was measured by three tests: the MTT reduction, LDH release and Neutral Red uptake. The apoptosis was determined by photometric enzyme- immunoassay. Moreover, the IL-1 and IL-6 concentration was determined.
The present study showed that chromium chloride decreases cells viability (confirmed in three cell viability tests) after 24 h of incubation. The suggested mechanism of chromium action in mouse embryo fibroblasts can be presented as follows: the chromium in cells interacts first with mitochondria, then with cell membrane and finally with lysosomes. Moreover, the observed increase of mono- and oligonucleosomes in the cytoplasm of cells is caused by the fact that DNA degeneration occurs several hours before the plasma membrane breakdown. Moreover, chromium chloride increases IL-1 and decreases IL-6 concentration secreted into the cell culture supernatant.
keywords:

chromium, cytotoxicity, apoptosis, cytokines

FEATURED PRODUCTS
Quick links
© 2019 Termedia Sp. z o.o. All rights reserved.
Developed by Bentus.
PayU - płatności internetowe