eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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2/2008
vol. 33
 
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abstract:

Experimental immunology
Cooperation between lysozyme and complement system in bactericidal action of human serum – is everything already clear?

Gabriela Bugla-Płoskońska, Adam Kiersnowski, Bożena Futoma-Kołoch, Włodzimierz Doroszkiewicz

(Centr Eur J Immunol 2008; 33 (2): 37-42)
Online publish date: 2008/05/05
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The complement system plays an important role in protection of higher organisms against bacterial infection. Lysozyme cooperates with the complement system in the bactericidal action of serum. Adsorption of serum onto bentonite (montmorillonite, MMT) is well known procedure to remove the lysozyme from serum. Our present results show that the most efficient killing of Salmonella O48 occurred when all components of normal human serum (NHS) cooperated with each other. It is very interesting that elimination of lysozyme from NHS by using MMT significantly decreased the bactericidal activity of NHS against Salmonella O48 strain. The results of X-ray diffractometric studies suggested that apart from lysozyme, other components of serum were adsorbed on the bentonite particles.
keywords:

lysozyme, montmorillonite, normal human serum, Salmonella

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